microRNA-130b在结肠癌中的表达及生物学意义研究

Expression and biological significance of microRNA-130b in human colonic carcinoma

目的:研究微小RNA 130b(microRNA-130b,miR-130b)在结肠癌(Colonic carcinoma,CRC)中的表达情况及生物学意义。方法:收集2013年1月至2015年3月于我院普通外科行手术切除的CRC组织及对应癌旁组织共60例,运用qRT-PCR技术检测miR-130b在CRC组织及癌旁组织中的表达水平,统计分析miR-130b表达水平与患者临床病理资料间的相关性;采用人工合成的miR-130b模拟物转染人结肠癌SW480细胞,分别采用CCK-8分析、Brd U检测转染后细胞增殖变化,流式细胞仪及Caspase3/7活性分析检测转染后细胞凋亡变化。结果:miR-130b在CRC组织中表达水平显著高于对应癌旁组织(P<0.05);miR-130b高表达与肿瘤体积增大(≥5 cm,P<0.05)及高TNM分期(Ⅲ+Ⅳ期,P<0.05)显著相关;在人结肠癌SW480细胞中过表达miR-130b可显著抑制细胞增殖并促进细胞凋亡(P<0.05)。结论:miR-130b在结肠癌组织中表达下调并与肿瘤恶性临床病理特征有关,miR-130b可能通过抑制结肠癌细胞增殖、促进凋亡来抑制结肠癌的生长及发展。

Objective： To investigate the expression and biological significance of microRNA-130 b in human colonic carcinoma（ CRC）. Methods： qRT-PCR was applied to detect the relative expression of miR-130 b in 60 paired colonic carcinoma in comparison to the tumor adjacent tissues. Student-t test was used to analyze the relationship between the miR-130 b expression and clinical features. We transfected the miR-130 b inhibitor into SW480 cells,CCK-8 and Brd U incorporation assay were used to analyze cell proliferation,and flow cytometry and caspase3 /7 activity assay were used to analyze cell apoptosis. Results： The relative expressions of miR-130 b was significantly down-regulated in CRC tissues compared to those of the matched normal tumor-adjacent tissues（ P〈0. 05）. Low expression of miR-130 b was significantly associated with large tumor size（ ≥5 cm,P〈0. 05） and advanced TNM stage（ Ⅲ＋Ⅳ,P〈0. 05）. Overexpression of miR-130 b in SW480 cells could significantly suppress cell proliferation and induce cell apoptosis（ P〈0. 05）. Conclusion：Low expression of miR-130 b is related to the malignant clinicopathological features in CRC tissues,and miR-130 b suppress colonic carcinoma growth and progression through inhibiting proliferation and enhancing apoptosis.