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右美托咪定对烫伤致大鼠急性肺损伤的影响 被引量:1

Effect of dexmedetomidine on scalds-induced acute lung injury in rats
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摘要 目的 评价右美托咪定对烫伤致大鼠急性肺损伤的影响.方法 雄性成年SD大鼠75只,体重200 ~ 220 g,采用随机数字表法分为5组(n=15):对照组(C组)、烫伤组(S组)、右美托咪定组(D组)、α-银环蛇毒素组(α-BGT组)、右美托咪定+α-银环蛇毒素组(D+α-BGT组).S组、D组、α-BGT组和D+α-BGT组将大鼠背部皮肤(约30%体表面积)脱毛后浸入98℃热水中12s制备烫伤模型;C组大鼠背部皮肤浸于37℃温水中12 s.造模后24 h内腹腔注射乳酸钠林格氏液进行容量复苏治疗.D组造模前15 min时腹腔注射右美托咪定40ug/kg;α-BGT组造模前15 min时腹腔注射α7型烟碱乙酰胆碱受体拮抗剂α-银环蛇毒素1μg/kg;D+α-BGT组造模前15 min时分别腹腔注射α-银环蛇毒素1 μg/kg和右美托咪定40 μg/kg.于造模后24 h时,处死大鼠,取肺组织,观察病理学结果,计算肺含水率,测定髓过氧化物酶(MPO)活性,采用ELISA法检测IL-1β、TNF-α及IL-6的含量,采用Western blot法检测核蛋白NF-κB的表达水平.结果 与C组比较,S组、α-BGT组和D组肺组织含水率、MPO活性、IL-1β、TNF-α及IL-6的含量升高,NF-κB表达上调(P<0.05);与S组比较,D组和D+α-BGT组肺组织含水率、MPO活性、IL-1β、TNF-α及IL-6的含量降低,NF-κB表达下调(P<0.05),α-BGT组上述指标差异无统计学意义(P>0.05),病理学损伤减轻;与D组比较,D+α-BGT组肺组织含水率、MPO活性、IL-1β、TNF-α及IL-6的含量升高,NF-κB表达上调(P<0.05),病理学损伤加重.结论 右美托咪定可减轻烫伤致大鼠急性肺损伤. Objective To evaluate the effect of dexmedetomidine on acute lung injury induced by scalds in rats.Methods Seventy-five male Sprague-Dawley rats,weighing 200-220 g,were randomly divided into 5 groups (n =15 each) using a random number table:control group (group C),scald group (group S),dexmedetomidine group (group D),α-bungarotoxin (α-BGT) group,and dexmedetomidine+ α-BGT group (group D+α-BGT).About 30% of the total body surface was shaved and then exposed to 98 ℃ water for 12 s in S,D,α-BGT and D+α-BGT groups.The back of rats was exposed to 37 ℃ water for 12 s in group C.Rats were resuscitated with lactated Ringer's solution injected intraperitoneally according to Parkland formula within 24 h after establishment of the model.In D,α-BGT,and D+α-BGT groups,dexrnedetomidine 40 μg/kg,α7 nicotinic acetylcholine receptor antagonist α-BGT 1 μg/kg,and α-BGT 1 μg/kg plus dexmedetomidine 40 μg/kg were injected intraperitoneally,respectively,at 15 min before establishnent of the model.At 24 h after establishment of the model,the rats were sacrificed,and lungs were removed for examination of the pathological changes and for determination of myeloperoxidase (MPO) activity,in terleukin-1beta (IL-1β),tumor necrosis factor-alpha (TNF-α),and IL-6 contents (by enzyme-linked immunosorbent assay),and nucleoprotein factor kappa B (NF-κB) (by Western blot).The lung water content [(wet weight-dry weight)÷wet weight×100%] was calculated.Results Compared with group C,the lung water content,MPO activities,and contents of IL-1β,TNF-α and IL-6 were significantly increased,and the expression of NF-κB was up-regulated in S,α-BGT and D groups (P〈0.05).Compared with group S,the lung water content,MPO activities,and contents of IL-1β,TNF-α and IL-6 were significantly decreased,and the expression of NF-κB was down-regulated in D and D+α-BGT groups (P〈0.05),and no significant change was found in the parameters mentioned above (P〉0.05),and the pathological changes were significantly attenuated in group α-BGT.Compared with group D,the lung water content,MPO activities,and contents of IL-1β,TNF-α and IL-6 were significantly increased,the expression of NF-κB was up-regulated (P〈0.05),and the pathological changes were aggravated in group D + α-BGT.Conclusion Dexmedetomidine can mitigate scalds-induced acute lung injury in rats.
出处 《中华麻醉学杂志》 CAS CSCD 北大核心 2016年第3期372-375,共4页 Chinese Journal of Anesthesiology
关键词 右美托咪啶 烧伤 呼吸窘迫综合征 成人 Dexmedetomidine Burns Respiratory distress syndrome,adult
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