土白术药材中(6E,12E)-十四癸二烯-8,10-二炔-1,3-二乙酸酯和芹烷二烯酮的定性定量方法研究

Qualitative and quantitative research of( 6E,12E)-Tetradecadiene-8,10-diyne-1,3-dioldiacetate and Selina-4( 14),7( 11)-dien-8-one in Atractylodis Japonicae Rhizoma

目的建立土白术药材中(6E,12E)-十四癸二烯-8,10-二炔-1,3-二乙酸酯(TDEYA)和芹烷二烯酮两种化学成分的定性定量方法。方法采用薄层色谱法和高效液相色谱法。薄层色谱采用G254薄层硅胶预制板,以石油醚-乙酸乙酯(10∶1)为展开剂,置紫外光(254 nm)下检视。高效液相采用ZORBAX SB-C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-1%甲酸溶液(67∶33),检测波长为254 nm,流速1.0 ml/min;柱温30℃;进样量10μl。结果 TDEYA和芹烷二烯酮在薄层色谱和高效液相色谱上均获得良好的分离,可作为土白术药材的定性定量指标。结论本法操作简单,结果准确,重现性好,可为评价土白术药材的质量标准提供检测方法。

Objective To use the polytene component（ 6E,12E）- Tetradecadiene-8,10-diyne-1,3-dioldiacetate（ TDEYA） and sesquiterpene component selina-4（ 14）,7（ 11）-dien-8-one in Atractylodis Japonicae Rhizoma as the control chemicals to establish a TLC qualitative method and a HPLC quantitive method.Methods TLC method： place samples and control chemicals on pre-coated TLC plate（ SIL G-25 UV254）; use petroleum ether-ethyl acetate = 10： 1 as development solvent. The TCL plate was observed under 254 nm UV lamp. HPLC method： ZORBAX SB-C_（18）（ 250 mm × 4. 6 mm,5 μm）; mobile phase：methanol-1% formic acid solution（ 67 ∶ 33）; samples were detected under 254 nm ultraviolet lamp; flow rate： 1. 0 ml / min; column temperature： 30 ℃; injection amount： 10 μl. Results The polytene component TDEYA and sesquiterpene component selina-4（ 14）,7（ 11）- dien-8-one were separated well by TLC and HPLC. This method could be used as the qualitative and quantitative methods of evaluating Atractylodis Japonicae Rhizoma. Conclusion This method is simple in operation,accurate in results and good in reappearance,and can be used as the detection method for evaluating the quality of Atractylodis Japonicae Rhizoma.