赶黄草的安全性毒理学评价研究

Toxicological Assessment on Safety of Penthorum chinense Pursh

目的评价赶黄草的食用安全性。方法依据食品安全性毒理学评价程序和方法进行大、小鼠急性经口毒性试验、遗传毒性试验(Ames试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验)、大鼠30 d喂养试验、大鼠致畸试验。结果大、小鼠急性经口MTD值均大于15 000 mg/kg.BW,属无毒级。3项遗传毒性试验结果未见赶黄草致突变作用。大鼠30d喂养试验结果显示,动物体重增重、进食量、食物利用率、脏体比值、血液学指标及末期血生化指标检测结果与阴性对照组比较,除雄鼠高剂量组嗜酸性细胞降低差异有统计学意义(F=2.885,P<0.05),其余各项指标差异均无统计学意义,对大鼠脏器组织均未观察到有害作用。大鼠致畸试验结果显示,赶黄草对大鼠未见母体毒性、胚胎毒性和致畸作用。结论受试赶黄草在本试验条件下未显示有急性毒性、遗传毒性、亚急性毒性或致畸作用。

Objective To evaluate the edible safety of Penthorum chinense Pursh. Methods The acute oral toxicity test of mice and rats,genetic toxicity tests（ including mice bone marrow cell micronucleus test,mice sperm abnormality test and Ames test）,30- day feeding test on rats and teratogenicity test were performed in accordance with the procedures and methods for toxicological assessment on food safety. Results The MTD for mice and rats was more than 15 000 mg / kg·BW,belonging to non- toxic grade. The negative results were obtained in genetic toxicity tests. In 30- day feeding test,there was no significant difference in weight increase,food intake,food utilization,organ coefficient,blood routine examination or blood biochemistry index between treatment group and control group（ P 0. 05）,except the significant decrease of acidophilic cell of high- dose male rats（P 0. 05）. There was no apparent pathological injury to the major organs of rats was observed in the pathological examination of rats ＇ tissues. No maternal toxicity,embryonic toxicity or teratogenicity had been found.Conclusion Penthorum chinense Pursh shows acute toxicity,no genetic toxicity,no subchronic toxicity and no teratogenicity in this study.