重庆地区部分静脉注射毒品者丙型肝炎病毒感染状况的调查

SURVEY OF HCV INFECTION IN INTRAVENOUS INJECTION DRUG ABUSERS IN CHONGQING

目的··:调查重庆地区部分入住我院的静脉注射毒品者丙型肝炎病毒(HCV)不同基因型感染状况。方法·· :用酶标记免疫(ELISA)法检测HCV感染情况 ,用逆转录 -聚合酶链反应(RT -PCR)与限制性长度多态性(RFLP)分型检测HCVRNA ,并观察血清ALT、AST水平。结果··:入住我院的静脉注射毒品者中抗 -HCVIgG阳性率为40.5 %;HCVRNA阳性率为34.5 %,其中HCV1b型、2a型及1b/2a混合型感染率分别为33.7 %、46.9 %及19.4 %。在ALT/AST正常者和异常者之间 ,HCV各类型感染率无显著性差异。结论··:重庆地区部分静脉注射毒品者HCV感染以2a型为主 ,其次为1b型 ,1b/2a混合型亦不少见。HCV基因型与血清ALT和AST水平变化无明显相关性。

Objective:To investigate the prevalence of HCV infection in intravenous drug users (IDUs) and molecular biological characteristics of HCV. Methods: The serum samples from 284 IDUs were obtained, and anti-HCV IgG was detected by ELISA, HCV was typed by RT-PCR and restriction fragment length polymorphism ( RFLP ) analysis of the 5' untranslated region.Results: There were 115 cases (40.5%) anti-HCV IgG positive and 98 cases( 34.5%) HCV-RNA positive in the 284 IDUs. The HCV 1b and HCV 2a genotypes and the 1b/2a mixed genotype accounted for 33.7%, 46.9% and 19.4% of HCV infection, respectively. The distribution of HCV genotypes in the individuals with different levels of ALT was identical. Conclusion: Infection of HCV 2a genotype was predominant in the IDUs in Chongqing, followed by HCV 1b and 1b/2a mixed genotypes. The different genotypes of HCV were not related to the change of serum ALT levels.