针刺联合胎盘间充质干细胞治疗脑性瘫痪大鼠模型

Combination of acupuncture with placenta derived mesenchymal stem cell transplantation for cerebral palsy in a rat model

背景:针刺和间充质干细胞移植均对脑性瘫痪有一定的治疗效果。目的:探讨针刺联合胎盘间充质干细胞移植对脑性瘫痪模型大鼠的治疗效果。方法:将35只Wistar大鼠随机分为对照组、模型组、针刺组、移植组和针刺联合移植组,除对照组外,其他4组建立脑性瘫痪模型。造模后7 d移植组大鼠在右侧纹状体区和大脑皮质下移植10μL胎盘间充质干细胞(1×1011 L-1);针刺组在水沟、大椎、百会、关元、承浆和气海6个穴位针刺治疗;针刺联合移植组按上述方法给予胎盘间充质干细胞移植和针刺治疗;模型组和对照组不做任何处理,干预7 d后,测量大鼠的体质量、神经功能,并观察脑组织的病理形态学变化。结果与结论:1大鼠的体质量增长率:对照组>针刺联合移植组>针刺组及移植组>模型组,针刺组和移植组大鼠的体质量增加相似,其他组间比较差异有显著性意义(P<0.05);2握持牵引、足错误实验和迷宫觅水实验结果:对照组>针刺联合移植组>针刺组、移植组>模型组,针刺组和移植组大鼠的神经功能改善相似,其他组间比较差异有显著性意义(P<0.05);3病理形态学变化:模型组大鼠大脑组织和海马区脑组织的细胞数量减少,排列紊乱,有炎性细胞聚集,细胞结构模糊。针刺组、移植组和针刺联合移植组大脑组织和海马组织病理改变介于对照组和模型组之间,其中针刺联合移植组的病理改变较针刺组和移植组好,有向正常大脑组织接近的趋势;4由此可见,单纯针刺、单纯胎盘间充质干细胞移植和针刺联合胎盘间充质干细胞移植均能改善脑性瘫痪大鼠的神经功能,其中针刺联合胎盘间充质干细胞移植治疗的效果最好。

BACKGROUND： Both of acupuncture and mesenchymal stem cell transplantation have therapeutic effects on cerebral palsy. OBJECTIVE： To explore the therapentic effect of acupuncture combined with placenta derived mesenchymal stem cells on rat cerebral palsy. METHODS： Thirty-five Wistar rats were equivalently randomized into five groups, including control group, model group, acupuncture group, transplantation group and combination group. Rat models of cerebral palsy were prepared in all groups except the control group. At 7 days after modeling, in the transplantation group, 10 μL placenta derived mesenchymal stem cells were transplanted into the right cerebral cortex and striatum of rats; rats in theacupuncture group were given acupuncture at Shuigou（DU26）, Dazhui（DU14）, Baihui（DU20）, Guanyuan（RN4） and Qihai（RN6） points; rats in the combination group given acupuncture and transplantation treatment as described above; the model and control groups received no treatment. Then, after 7-day intervention, the body mass, neurological function and pathological changes of rat brain tissues were measured and observed, respectively.. RESULTS AND CONCLUSION： The order of the growth rate of body mass was as follows： control group combination group acupuncture and transplantation groups model group, and all groups had significant differences except the two groups of acupuncture and transplantation（P〈0.05）; findings from holding tract, foot error and water finding in maze tests showed the neurological function was best in the control group, followed by the combination group, as well as acupuncture and transplantation groups, and the worst in the model group. All groups had significant differences except acupuncture and transplantation groups（P〈0.05）. Besides, in the model group, cells in the brain and hippocampus decreased, arranging disorderly with fuzzy structure, and inflammatory cells appeared; pathological changes in the other groups were in between control group and model group, Additionally, brain tissues in the combination group exlibited better pathological changes than those in the acupuncture and transplantation groups, which were similar with the normal brain tissues. To conclude, acupuncture, placenta derived mesenchymal stem cells transplantation, or their combination can improve neurological function in rats with cerebral palsy, especially the combination treatment.