不同体质MRL/lpr狼疮鼠病情程度及Treg/Th17相关细胞因子表达

Correlation Between Severity and Expression of Treg / Th17-relating Cytokines in MRL / lpr Lupus Mice with Different Constitution

目的:区分MRL/lpr狼疮鼠体质,观察不同体质狼疮鼠病情程度及细胞因子水平的差异。方法:将12周龄MRL/lpr狼疮鼠按体质分为寒体组、常体组、热体组。16周龄时,采用CBB法酶标仪检测24 h尿蛋白;HE、PAS、PASM、MASSON染色后观察肾脏组织病理;双抗体夹心ABC-ELISA法检测不同组别狼疮鼠血清细胞因子(IL-6、IL-10、IL-17、IL-23、TGF-β1)水平。结果:16周龄时,寒体组MRL/lpr狼疮鼠24 h尿蛋白定量高于常体组和热体组(P<0.05),寒体组肾脏组织病理表现相对严重;寒体组IL-6、TGF-β1表达水平高于常体组和热体组(P<0.01),IL-10表达低于常体组和热体组(P<0.01),热体组IL-23表达水平高于常体组和寒体组(P<0.01),各组间IL-17表达差异无统计学意义(P>0.05)。结论:MRL/plr狼疮鼠存在体质差异,寒体组肾脏病变程度在3组中最为严重,不同体质狼疮鼠血清细胞因子水平存在差异。

Objective： To identify the constitution of MRL / lpr lupus mice and observe the difference of disease severity and cytokines of MRL / lpr lupus mice with different constitutions. Methods： 12-week MRL / lpr lupus mice were classified into cold,normal and hot constitutions. After fed for four weeks,expressions of serum IL-6,IL-10,IL-17,IL-23,TGF-β1,24 h urinary protein and renal pathology were examined. Results： In 16-week MRL / lpr lupus mice,24 h urinary protein in cold body group were higher than normal and hot groups（ P〈0. 05）; the kidney pathological changes of cold body group were relatively serious; the expressions of serum IL-6,TGF-beta1 of cold body group were higher than normal and hot body groups（ P〈0. 01）; the expression of IL-10 in cold body group was less than normal and hot body groups（ P〈0. 01）; IL-23 expression of hot body group was higher than normal and cold groups（ P〈0. 01）,but there was no difference of IL-17 expression between three groups（ P〈0. 05）. The expressions of serum IL-6,IL-10,IL-23 and TGF-β showed significant differences（ P〈0. 01）. Conclusion： There are differences in the constitution of MRL / lpr lupus mice and the level of serum cytokines. The kidney injury of cold constitution group was the most serious.