速测盒法快速检测果蔬中拟除虫菊酯类农药残留

Rapid detection of pyrethroid pesticide residues in fruits and vegetables by test kit

目的了解菊酯类农药残留速测盒的可靠性及实用性。方法采用速测盒法检测拟除虫菊酯类农药标准溶液和果蔬样品中拟除虫菊酯类农药残留情况,并与气相色谱-质谱联用法进行对比;同时进行批间验证及同一批次在不同环境温度下的比对试验。结果速测盒法对8种常用拟除虫菊酯类农药最低检出限均可达到0.50 mg/kg以下;5个批次的速测盒显色结果基本一致,批次间重现性较好。在环境温度为5℃～40℃时,速测盒1 min内即可显色完全,且30 min内不褪色,受环境温度影响小。速测盒法与气相色谱-质谱联用法的阴性符合率为97.8%,阳性符合率为85.7%。结论速测盒法具有快速、准确、方便、灵敏等特点,适用于在现场对果蔬中拟除虫菊酯类农药残留进行定性分析。

Objective To know the reliability and practicability of test kit for quick detection of pyrethroid pesticide residues in fruits and vegetables. Methods Pyrethroid pesticide residues in fruits and vegetables were examined by the test kit and gas chromatography- mass spectrometry respectively,and the results were compared with each other. Also comparison was conducted among different batches,and in the same batch in different temperatures. Results The minimum detection limit of 8 kinds of pyrethroid pesticide with test kit was lower than 0. 5 mg / kg. 5 batches of test kit result were basically the same,a good reproducibility between batches were showed. The test kit can be colored in 1 min,and didn＇t fade within 30 min,while the temperature was among 5 ℃- 40 ℃,showing that temperature had bare effect on the test kit. 97. 8% of positive results as well as85. 7% of negative results by the test kit were coincident with those by gas chromatography mass spectrometry. Conclusion The test kit is rapid,accurate,convenient,and suitable for the quick site detection and identification of pyrethroid pesticide in fruits and vegetables.