辽宁省首次实验室确诊1例先天性风疹综合征患儿

The first laboratory diagnosis of a congenital rubella syndrome child in Liaoning

目的首次实验室确诊1例先天性风疹综合征(congenital rubella syndrome,CRS)患者,为CRS的快速诊断和控制提供科学依据。方法采集患儿的血清、咽拭子和尿液标本,分别进行风疹IgM抗体检测和风疹病毒(RV)分离。应用逆转录PCR(RT-PCR)扩增RV分离株E1基因的945个核苷酸片段,并对扩增产物进行序列测定和分析。结果患儿的血清风疹IgM抗体阳性。从患儿的咽拭子和尿液中均分离出RV,通过比较核苷酸和氨基酸同源性及构建基因亲缘关系树,发现本例CRS的RV分离株属1E基因型,与1E基因型参考株(RVI MYS 01 1E)序列的核苷酸和氨基酸同源性分别为98.4%和99.6%。结论辽宁省首次通过实验室诊断方法对CRS临床符合病例进行确诊,并分离出1E基因型风疹病毒。

Objective Laboratory method was used for the diagnosis of a case of congenital rubella syndrome（ CRS） in children,so as to provide scientific basis for the rapid diagnosis and control of CRS. Methods Serum,throat swab and urine specimens were collected from CRS children respectively for the detection of rubella IgM antibody and the isolation of rubella virus（ RV）. In the study,the fragments containing 945 nucleotides of E1 genes from rubella virus isolates were amplified by RT-PCR method,and the PCR products were sequenced and analyzed. Results The rubella IgM antibody was positive. Rubella virus were isolated from children patients＇ throat swab and urine. By comparing the homology between nucleotide and amino acid and constructing phylogenetic tree,it was found that the RV isolate of this CRS case belongs to 1E genotype,and its nucleotide and amino acid homology with genotype 1E reference strain（ RVI MYS 01 1E） sequences were 98. 4% and 99. 6%.Conclusion The CRS clinical case was diagnosed through laboratory diagnostic method for the first time in Liaoning,and the1 E rubella virus was isolated.