青枯雷尔氏菌胞外蛋白指纹多态性分析

Polymorphism analysis of extracellular protein from Ralstonia solanacearum

采用SDS-PAGE技术对70株不同来源及致病力青枯雷尔氏菌(Ralstonia solanacearum)进行胞外蛋白指纹多态性分析,研究结果表明,供试青枯雷尔氏菌菌株呈现丰富的胞外蛋白指纹多态性,多态性比率为100%。不同来源青枯雷尔氏菌分泌的胞外蛋白不同,EZ-Tn5^(TM)插入诱变菌株电泳出20条不同大小蛋白条带,分子量集中在20~97 kD,且菌株间蛋白条带相似或完全相同;^(60)Co辐射诱变菌株共电泳出16条不同大小的蛋白条带,多数蛋白分子量44.3 kD;野生型菌株电泳的蛋白条带最多,共获得26条不同大小的蛋白条带。进一步对37株不同致病力的野生型菌株进行胞外蛋白含量测定,结果表明,不同致病力菌株胞外蛋白含量差异大,强致病力菌株分泌的胞外蛋白含量较高,为1.026~5.963μg/mL,无致病力菌株胞外蛋白含量较低,为0.083~0.761μg/mL。

By using SDS-PAGE,the extracellular protein polymorphism of 70 Ralstonia solanacearum wildtype and mutant strains were analyzed.The tested strains possessed abundant variation highly associated with their origins.Twenty protein bands were detected in the EZ-Tn5 transponson-generated mutants with a range molecular weights from 20-97 kD,and all mutants shared a highly similar protein band profile.The strains from^60Co-radiated mutagenesis produced 16 protein bands,most of which had molecular weight lower than 44.3 kD.The wild-type strains isolated from diseased plant samples produced the most abundant extracellular proteins（26bands）.Furthermore,extracellular protein concentrations of 37 wild-type R.solanacearum strains with different pathogenicity were determined.The virulent strains produced higher contents of extracellular proteins（1.026 to5.963 μg/mL） than that of avirulent strains（0.083 to 0.761μg/mL）.