摘要
目的观察中药筋脉通对链尿佐菌素(STZ)诱导的糖尿病(DM)大鼠背根神经节(DRG)解耦联蛋白3(UCP3)和胰岛素样生长因子(IGF-1)的表达的影响。方法将大鼠随机分为正常对照组、糖尿病模型组(腹腔内注射STZ、筋脉通及牛磺酸治疗组)。成模后每天1次灌胃给药,持续16周。电子Von Frey仪检测机械痛阈值,免疫组化法和real time-PCR法分别检测DRG中UCP3和IGF-1蛋白及mRNA的表达。结果与正常对照组相比,糖尿病大鼠出现机械痛阈值下降,DRG中UCP3和IGF-1蛋白及mRNA表达水平明显下降(P<0.01);中药筋脉通组可改善糖尿病大鼠周围神经痛阈下降(P<0.01),并且能够使DRG中UCP3和IGF-1蛋白及mRNA表达水平显著回升(P<0.01)。结论中药筋脉通可通过增加DRG神经组织中UCP3含量,抑制氧化应激对背根神经的损伤,并通过上调神经营养因子IGF-1的含量,促进糖尿病大鼠周围神经修复能力,而改善其痛觉异常。
Objective To study the effects of Jinmaitong on expression of UCP3 and IGF-1 in dorsal root ganglion（ DRG） of STZ-induced diabetic rats. Methods The STZ-induced diabetic rats were randomly divided into 4 groups including normal control group（ Con）,diabetic group（ DM）,JMT group（ JMT） and taurine group（ Tau） with six rats in each group. All rats were given intragastric administration for 16 weeks and then culled. The mechanical withdrawal threshold was tested by electronic Von Frey instrument before death. Immunohistochemical and q-PCR method was applied to detect the expression of UCP3 and IGF-1 in dorsal root ganglion. Results Compared with the control group,the mechanical pain threshold in the DM group significantly decreased（ P〈0. 01）. The protein level and the level of mRNA expression of UCP3 and IGF-1 in DM group were much lower than that in control group（ P〈0. 01）. JMT significantly improved mechanical pain threshold（ P〈0. 01）. Compared with DM group,the protein level and the level of mRNA expression of UCP3 andIGF-1 significantly increased（ P〈0. 01）. Conclusions JMT stimulates neuroprotection as well as neuroregeneration through up-regulating the expression of UCP3 and IGF-1 in DRG.
出处
《基础医学与临床》
CSCD
2016年第6期811-816,共6页
Basic and Clinical Medicine
基金
北京市自然科学基金(7122147)
