Cdc42基因在阿萨希毛孢子菌酵母相和菌丝相的表达差异

The Differences of Cdc42 Expression between the Yeast and Hyphal Forms of Trichosporon Asahiis

目的研究细胞分裂周期蛋白42(cell division cycle42,Cdc42)基因在阿萨希毛孢子菌的菌丝相和酵母相中的表达差异,探讨其在菌丝生长过程中的作用。方法分别培养标准株酵母相和菌丝相组、标准株不同时间生长组、4株不同来源菌株生长组,检测三组阿萨希毛孢子菌菌丝生成率,并提取总RNA,采用荧光定量PCR方法测定各组中Cdc42的基因表达,并计算其相对表达量。结果不同条件下菌丝生成率不同,Cdc42在各组中均有表达,且随着菌丝生成率的升高,Cdc42基因表达量基本呈上升趋势。结论 Cdc42参与菌丝的形成。

Objective To investigate the role of gene expression of Cdc42 （ cell division cycle42） in hyphal formation by detecting the differences of Cde42 mRNA expression between yeast and hyphal forms of Trichosporon asa- hiis. Methods Three groups under different conditions were set up： one group including two subgroups of yeast and hyphal forms of type strain, one group including four subgroups of different growth times of type strain and one group including four subgroups of four strains of T. asahii isolated from different sources. Ex- tracted the total RNA separately and measured the mRNA expression of Cdc42 with fluorescent quantitative PCR assay, then calculated the relative expression of mRNA. Results Different conditions resulted in dif- ferent hyphal formation rates and Cdc42 expressed in all groups. With the increase of the rate, the expres- sion of Cdc42 was almost increasing. Conclusion Cdc42 participates in the regulation of hyphal formation in T. asahii.