膀胱癌组织端粒酶活性研究

Telomerase activity in human bladder carcinoma

目的 :探索端粒酶活性与膀胱癌发生发展的关系。方法:采用TRAP_PCR_ELISA方法检测39例膀胱癌组织、25例癌旁组织及10例正常膀胱组织的端粒酶活性。结果:膀胱癌组织中端粒酶表达阳性率为89 7 %(35/39) ;癌旁组织中端粒酶表达阳性率为20%(5/25) ;正常组织中无端粒酶活性表达。结论:端粒酶活性与膀胱癌发生发展密切相关 。

Objective:To study the possible relationship between telomerase activity and progression of human bladder carcinoma.Methods:Telomerase activity was examined by TRAP_PCR_ELISA in tissue sample which were taken from 39 bladder carcinoma,25 adjacent noncancerous bladders and 10 normal bladders.Results:The positive rate of telomerase acitivity was 89.7%(35/39),20%(5/25)and 0%(0/10)in bladder carcinoma,in adjavent noncancerous bladder and in normal bladder respectively.Conclusion:Telomerase activity might contribute to progression of bladder carcinoma and could perhaps be a useful tumor marker for the detection of early recurrent bladder carcinoma.