电针对缺血性脑卒中大鼠运动功能CatWalk行为学的影响

Effect of electroacupuncture on motor function analyzed by Cat Walk gait system in cerebral ischemic rats

目的:研究电针对缺血性脑卒中大鼠运动功能Cat Walk行为学相关参数的影响。方法:采用改良线栓法制备大鼠大脑中动脉闭塞再灌注模型。雄性SD大鼠随机分成3组:假手术组、模型组、电针组。对模型组、电针组大鼠在电针干预前后进行神经行为学评分;同时在电针干预7d后,分别对3组大鼠进行CatWalk步态分析系统的行为学检测。来探讨电针治疗缺血性脑卒中大鼠运动功能的有效性,以及Cat Walk步态分析系统可以作为评价电针改善运动功能的可行性。结果:电针治疗7d后,电针组与模型组的神经行为学评分有显著性差异(P<0.05);而Cat Walk步态分析系统结果显示从大鼠的运动速度、持续时间和每一只爪子的爪印面积、最大接触面积、最大压强、平均压强、步长等不同客观参数,显示了模型组与假手术组之间的差异(P<0.05),以及电针组与模型组之间的差异。结论:电针改善缺血性脑卒中大鼠运动功能Cat Walk行为学,促进脑功能恢复。Cat Walk步态分析系统可以精确、客观地反映出大鼠运动功能的变化,对于电针对大鼠行为学实验研究有重要意义。

Objective： To study the effect of electroacupuncture（EA） treatment on the Cat Walk behavior parameters relevant with the motor function in rats with ischemic stroke.Method： Middle cerebral artery occlusion（MCAO）-reperfusion model was established by modified thread-occlusion method. Male SD rats were randomly divided into three groups： sham operation group, model group, and EA group. In the model group and EA group, we scored the neurobehavioral function before and after intervention. At 7d after intervention, three groups were given behavioral assessment using the Cat Walk gait analysis system. We evaluated the effectiveness of the electroacupuncture and the feasibility of the Cat Walk gait analysis system.Result： For the neurobehavioral score, there was a significant difference between the EA group and the model group after 7d treatment（P〈0.05）. The results from Cat Walk gait analysis system showed the differences in motor speed, duration, each paw print area, the maximum contact area, the maximum intensity, mean intensity,stride length between the model group and the EA group or the sham group（P〈0.05）.Conclusion： The EA improves Cat Walk behaviors of motor function and brain function in rats with ischemic stroke. The Cat Walk gait analysis system could reflect the changes in motor function in rats accurately and ob-jectively, which has important significance for the research on the electroacupuncture effect on the behavior of rats.