山参磷脂氢谷胱甘肽过氧化物酶cDNA克隆及序列分析

Cloning and sequence analysis of wild ginseng phospholipid hydroperoxide glutathione peroxidase cDNA

磷脂氢谷胱甘肽过氧化物酶(PHGPx)具有明显的抗氧化活性,避免细胞膜受到过氧化损伤。本研究利用RACE技术,克隆出山参phgpx基因全长c DNA。序列分析表明:该克隆片段全长490 bp,编码162个氨基酸,有3个真核生物PHGPx特有的保守亚结构域。该片段编码的蛋白为跨膜亲水性稳定蛋白质,与柑橘、蓖麻等PHGPx氨基酸序列同源性分别为76%和75%。同时建立了9种植物的系统进化树。

Phospholipid hydroperoxide glutathione peroxidase（PHGPx） has significant antioxidant activity, avoiding the harm by peroxidation in the cell membrane.To clone the full-length cDNA encoding PHGPx gene from Panax ginseng,the RACE technology was used in this study.The sequence analysis showed that： the full length of the cloned fragment was 490 bp.lt encoded 162 amino acids and contained 3 sub-conservative domains which were specific in eukaryonic phospholipid hydroperodixe glutathione peroxidase.The encoding protein was a stable non- transmembrane hudrophilic protein.The sequence of amino acids was similar with PHGPx of citrus and castor, homologous rates of amino acids were 76% and 75%. And then, the phylogenetic tree of nine plants was constructed.