摘要
【目的】了解登革热患者血小板膜糖蛋白特异性抗体(抗GPIIb/IIIa和抗GPIb/IX)的表达,探讨登革热患者血小板减少是否与血小板膜糖蛋白特异性抗体相关。【方法】用改良的血小板抗原单抗特异性固相化法(MAIPA法)检测51例登革病毒1型(DENV-1)登革热患者和50例健康者血清的抗GPIIb/IIIa和抗GPIb/IX特异性抗体,分析血小板膜糖蛋白特异性抗体水平和血小板计数的相关性。【结果】51例DENV-1登革热患者血清中血小板膜糖蛋白特异性抗体抗GPIIb/IIIa、抗GPIb/IX和双抗体总体阳性率分别为35.3%(18/51)、37.3%(19/51)和43.1%(22/51);与50例健康者的阳性率8.0%(4/50),6.0%(3/50)和10.0%(5/50)相比,差异有统计学意义(P<0.001)。重度血小板减少登革热患者(PLT<50×109/L)血清中,抗GPIIb/IIIa和抗GPIb/IX抗体检出率为100%(6/6)和83.3%(5/6),血小板计数无减少登革热患者血清中抗体阳性率为14.8%(4/27)和29.6%(8/27),差异有统计学意义(P=0.0001,P=0.015)。在登革热血小板计数减少患者血清中,抗GPIIb/IIIa和抗GPIb/IX抗体水平与血小板计数呈负相关(r=-0.58,P<0.001;r=-0.44,P<0.05)。【结论】登革热患者血小板减少与血小板膜糖蛋白特异性抗体抗GPIIb/IIIa和抗GPIb/IX相关;免疫因素参与登革热患者血小板减少的破坏过程。
[Objective] To investigate the expression of platelet membrane glycoprotein specific antibodies (anti-GPIIb/IIIa and anti-GPIb/IX) in the patients with dengue fever, it was explored that whether thromboeytopenia in dengue fever was correlated with specific antibody against platelet membrane glycoprotein. [ Methods ] A modified monoclonal antibody specific immobilization of platelet antigens (MAIPA) method was used to detect the anti-GPIIb/IIIa and anti-GPIb/IX in the sera of 51 DENV-1 dengue fever patients and 50 healthy volunteers. The correlation between the antibody and platelet count was analyzed. [ Results ] The positive rate of anti-GPIIb/IIIa, anti-GPIb/IX and general positive rate were 35.3% (18/51), 37.3% (19/51) and 43.1% (22/51) respectively in 51 DENV-I dengue fever patients. Compared with the antibody positive rate of 50 healthy volunteers [8.0% (4/50), 6.0% (3/50) and 10.0% (5/50) respectively], the differences were statistically significant (P〈0.001). The positive rate of anti-GPIIb/IIIa and anti-GPIb/IX in dengue fever patients with severe thrombocytopenia (PLT 〈 50 × 109/L) were 100% (6/6) and 83.3% (5/6). Compared with the antibody positive rate of dengue fever patients without thrombocytopenia [ 14.8% (4/27) and 29.6% (8/27)], the differences were statistically significant (P = 0.0001; P = 0.015). The anti-GPIIb/IIIa and anti-GPIb/IX antibody levels were negatively correlated with platelet count in dengue fever patients with thrombocytopenia (r = -0.58, P 〈 0.001 ; r = -0.44, P 〈 0.05). [ Conclusion ] Thrombocytopenia in dengue fever was correlated with specific antibody against platelet membrane glycoprotein. Immune thrombocytopenia was involved in the destruction of platelet of dengue fever patients.
出处
《中山大学学报(医学科学版)》
CAS
CSCD
北大核心
2016年第3期326-332,共7页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省科技计划项目(2013A020229001
2013A020229002)
关键词
登革热
登革病毒1型
血小板减少
血小板膜糖蛋白
血小板抗体
dengue fever
dengue virus type i
thrombocytopenia
platelet membrane glycoprotein
platelet antibody