葫芦素B对人胃癌BGC-823细胞增殖及凋亡的影响

Effects of cucurbitacin B on proliferation and apoptosis of human gastric carcinoma BGC-823 cells

目的探讨葫芦素B对人胃癌BGC-823细胞增殖及凋亡的影响及其作用的分子机制。方法体外培养BGC-823细胞,MTT法观察葫芦素B对细胞增殖的影响,采用流式细胞术检测细胞凋亡率,采用分光光度法检测Caspase-3、Caspase-9的活性,采用Western blot方法检测葫芦素B对Bcl-2、Bax蛋白表达的影响。结果葫芦素B对BGC-823细胞的增殖有抑制作用,且此作用呈剂量和时间依赖性;流式细胞仪检测结果显示,葫芦素B诱导BGC-823细胞凋亡,呈剂量依赖性。葫芦素B处理后Caspase-3、Caspase-9活性升高,细胞抗凋亡蛋白Bcl-2表达减少,促凋亡蛋白Bak的表达增加。结论葫芦素B可以抑制人胃癌BGC-823细胞的增殖并诱导细胞凋亡。

Objective To investigate the effects of cucurbitacin B on the proliferation and apoptosis of human gastric carcinoma BGC-823 cells, and to clarify the molecular mechanism. Methods The BGC-823 cells were cultured in vitro. MTT assay was used to test the growth inhibitory effect of cucurbitacin B on BGC-823 cells. Apoptosis rate was determined by flow cytometry analysis. Spectrophotometric method was used to determine the activity of Caspase-3 and Caspase-9. The expression of Bcl-2 and Bax was determined by Western blot after cucurbitacin B treatment. Results The growth of BGC-823 cells was inhibited by cucurbitacin B in a dose-and time-dependent manner. Flow cytometry analysis showed that cucurbitacin B could induce BGC-823 cells apoptosis in a dose-dependent manner. The activity of Caspase-3 and Caspase-9 was increased after treatment with cucurbitacin B. The anti-apoptotic protein Bcl-2was down-regulated, and the pro-apoptotic protein Bak was up-regulated. Conclusion Cucurbitacin B can inhibit the growth of BGC-823 cells and induce cell apoptosis.