摘要
以光甘草定为原料,采用复凝聚法制备光甘草定脂质体,以包封率和粒度为指标,采用正交试验设计优化光甘草定脂质体的制备工艺;并对光甘草定脂质体的粒径分布、体外释药特性和稳定性进行了研究。结果表明:光甘草定脂质体制备的最佳条件为卵磷脂与胆固醇质量比4∶1、超声波乳化时间40 min、光甘草定-丙二醇液质量浓度8 g/L,此条件下制得的脂质体粒径分布均匀,粒径为0.1-1.2μm的比例为84.67%,包封率和粒度综合评分可高达78以上。光甘草定脂质体前120 min的体外释药性优于光甘草定粉末,3个月时光甘草定质量分数仍达38.5%,保留率为96.25%。复凝聚法制备光甘草定脂质体工艺简单,产品稳定性好。
The glabridin liposome were prepared by the complex coacervation method with glabridin as raw material. Taking the encapsulation rate and particle size distribution as the indexes,the preparation technology of glabridin liposome was optimized by orthogonal design. The particle size distribution,release characteristics in vitro and stability of liposome were studied. The results showed that the optimal preparation conditions were the mass ratio of lecithin and cholesterol 4 ∶ 1,ultrasonic emulsific time40 min,and mass concentration of glabridin propylene glycol solution 8 g / L. Under these conditions,the obtained glabridin liposome had uniform particle size distribution and the percentage of particle size 0. 1-1. 2 μm was 84. 67 %. The comprehensive score of the encapsulation rate and particle size was over 78. The release charactistic of liposome was better than that of glabridin powder in the first 120 min and the mass fraction of glabridin at 3 months was 38. 5 % with the retention rate of 96. 25 %. Complex coacervation used to prepare glabridin liposome was simple and reliable,and the product was stable. As a new natural additive for whitening cosmetics,glabridin liposome had certain marketing significance.
出处
《生物质化学工程》
CAS
北大核心
2016年第3期8-12,共5页
Biomass Chemical Engineering
关键词
光甘草定
脂质体
复凝聚法
制备工艺
体外释放
glabridin
liposome
complex coacervation method
preparation technology
in vitro release