雷公藤红素对单核／巨噬细胞在舌癌中的影响

目的探讨雷公藤红素（Tri）对人单核／巨噬细胞系（THP-1细胞）和舌癌细胞（Tca8113）的影响。方法在酸性微环境中，加入不同浓度的Tri分别与Tca8113细胞、THP-1细胞＋Tca8113细胞、TH-1细胞进行培养24h、48h、72h后，MTT检测州在不同的浓度下，THP-1细胞对Tca8113的杀伤作用。结果THP-1细胞单独培养，随着Tri浓度的升高，细胞数量先增加后减少；THP-1细胞＋Tca8113细胞混合培养以及Tca8113细胞的单独培养，随着Tri浓度的升高，细胞数量逐渐减少，与浓度成负相关（r=-0．452，P〈0．05）。结论Tri在低浓度时，能促进THP-1细胞杀Tca8113细胞的作用，但浓度过大时，则有明显的杀伤THP-1细胞的作用。

Objective To study the effect of tripterine for the monocytes / macrophages （ THP-1 ） and the common culture of tongue carcinoma （ Tca8113 ） cells in vitro acid mi Methods In vitro microenvironments, different concentrations tripterine were common cultured with Tca8113 cell, Tca8113 cell＋ THP-1 cell, THP-1 cell after 24 hours, 48 hours, 72 hours, by MTT method to detected change of the number of tongue cancer cells and monocytes / macrophages after to intervente the detection of different tripterine conditions. Results when the monocytes / macrophages were separately cultured, via interfered by different densities of tripterine, the cell counts of Monocytes / macrophages increased and after to reduce, when the monocytes / macrophages and oral squamous cell carcinoma were mixed to culture, and oral squamous cell carcinoma were separately cultured, via interfered by different densities of tripterine, counts of cells was downward trending, and presented significantly inactive related to the densities of tripterine （ r=-0.452, P〈0.05 ） . Conclusion When the concentration of tripterine is lower, the tripterine can promote the increasing of THP-1 cells, however, when the concentration of tripterine is higher, the tripterine can kill the THP-1 cells.