3种免疫诊断试剂检测人群血清中日本血吸虫抗体的效果比较

Effect comparison of three immune-assays for the detection of Schistosoma japomicum antibody in human serum

目的评价斑点免疫金渗滤法(]DIGFA)、胶体染料法(DDIA)和间接血凝试验(IHA)三种方法在我县不同流行程度、不同流行类型地区中检测人群血清中血吸虫抗体的效果。方法按照整群抽样的方法,分别在洲滩型传播控制地区的群心村、山丘型传播控制地区的金塔村和传播阻断地区的郎坑村各随机抽取150人作为检测对象,分别用DIGFA、DDIA和IHA检测待检对象血清中血吸虫抗体,并对检测结果进行统计分析。结果 DIGFA、DDIA和IHA对传播阻断村人群血清均未检出血吸虫抗体;对山丘型传播控制村人群血清血吸虫抗体检测阳性率分别为13.3%(20/150)、2.0%(3/150)和12.0%(18/150);而三种方法组合检测的阳性率提高至19.33%(29/150),用DIGFA和IHA组合检测的阳性率提高至17.33%(26/150)。对洲滩型传播控制村人群血清血吸虫抗体检测阳性率,DIGFA、DDIA和IHA分别为26.7%(40/150)、9.3%(14/150)和24.7%(37/150);而三种方法组合检测的阳性率提高至36.0%(54/150),用DIGFA和IHA组合检测的阳性率提高至34.0%(51/150)。结论由于铜陵县疫情已达到传播控制,人群感染率和感染度逐年下降,采用多种方法组合检测,可以提高人群血吸虫抗体的检出率。

Objective To evaluate the effect of dot immunogold filtration assay（DIGFA）,colloidal dye method（DDIA） and indirect hemagglutination test（IHA） for the detection of Schistosoma japonicum antibody in serum of residents in different endemic degree areas and in different endemic type areas in Tongling County.Methods According to the method of cluster sampling,three villages were selected as study fields,namely Qunxin Village which was located in islet endemic areas and reached schistosomiasis transmission control criterion,Jinta Village which was located in hilly endemic areas and also reached schistosomiasis transmission control criterion,and Langkeng Village which reached schistosomiasis transmission interruption criterion.150 residents were randomly selected in each village,and DIGFA,DDIA and IHA were used to detect the S.japomicum antibody in serum of the residents,and the detect results were analyzed.Results By using DIGFA,DDIA and IHA to detect S.japonicum antibody in serum of residents,respectively,none positive were found in Qungxin Village,while the positive rate of of Jinta Village was 13.3%（20/150）,2.0%（3/150） and 12.0%（18/150）,respectively,and the positive rate of Qunxin Village was 26.7%（40/150）,9.3%（14/150） and2 4.7%（37/150）,respectively.By combined using DIGFA,DDIA and IHA to detect S.japomicum antibody in serum of the residents,the positive rate of Jinta Village and Qunxin Village was increased to 19.33%（29/150） and 36.0%（54/150）,respectively.By combined using DIGFA and IHA to detect S.japomicum antibody in serum of the residents,the positive rate of Jinta Village and Qunxin Village was increased to 17.33%（26/150） and 34.0%（51/150）,respectively.Conclusion Since Tongling County has reached schistosomiasis transmission control criterion,the infection rate and the infection degree of schistosomiasis in the residents are dropping year by year,combined using different methods can improve detection rate of S.japonicum antibody in serum of the residents.