摘要
利用野生大豆ZYD00006和栽培大豆绥农14所构建的回交导入系群体BC3F3代为研究材料,选择亲本间存在差异的121个SSR标记对114个株行材料进行基因型分析;利用T测验对含野生大豆纯合双导入位点(即B-B位点组合)植株表型值与绥农14表型值进行检测,以P≤0.05作为阈值,共获得104对B-B位点对表型影响显著,再经T测验,28对互作位点存在上位性效应,其中正向上位性效应位点10对,负向上位性效应位点18对,本研究结果揭示了上位性效应对大豆蛋白质含量性状的重要影响,同时这些位点信息将为大豆高蛋白分子辅助育种研究提供重要的理论基础。
The wild soybean backcross introgressive lines BC_3F_3 was constructed with cultural soybean Suinong 14 as recurrent parent and wild soybean ZYD00006 as donor parent in this study. One hundred and twenty one polymorphism SSR markers between the parents were used to analyze the genotypes of 114 lines. The line with double introgression homozygote loci from wild soybean plants（ B-B loci） and Suinong 14 were analyzed with T-test. With P≤0. 05 as threshold value,108 B-B locus were significant on phenotype. And epistasis effect was found at 28 B-B locus. Among them,10 were positive and 18 were negative.The result of this study have revealed the importance of epistasis effect on soybean protein content,meanwhile the information of these locus will be the theoretical basis for high protein of soybean breeding.
出处
《大豆科学》
CAS
CSCD
北大核心
2016年第3期353-359,共7页
Soybean Science
基金
黑龙江省新世纪优秀人才项目(1252-NCET-004)
关键词
野生大豆
回交导入系
蛋白质含量
上位性分析
Wild soybean
Backcross introgressive lines
Protein content
Epistasis analysis
