大豆花叶病毒病N1株系抗性基因定位分析

Mapping of Gene with Resistance to Soybean Mosaic Virus Strain N1

对大豆花叶病毒病N1株系不同抗性材料东农93046(抗)与品1246(感)所衍生的F8∶9代群体进行成株抗性鉴定,同时利用分子标记对其抗性基因进行确认并获得用于分子辅助选择的分子标记。结果表明:F8∶9代抗病家系数与感病家系数基本符合1∶1的比例,说明东农93-046对N1株系的成株抗性表现为质量性状,其抗性受一对等位基因控制。同时,根据前人研究结果利用76个SSR分子标记对父母本进行筛选,构建了一个包括13个SSR分子标记的F连锁群的遗传连锁图谱,并且单标记分析法和复合区间分析法的结果均表明东农93-046抗性基因位点位于SSR分子标记Satt114附近,对后代群体中抗病和感病株系各60份进行分子标记准确性评价,结果表明Satt114选择准确率可达80.00%,这为分子标记辅助选择奠定了良好的基础。

Soybean mosaic virus（ SMV） is one of the most widely distributed viral disease in soybean producing areas,which seriously impact on soybean yield and quality. Molecular marker assisted breeding was one of the most effective methods to select a resistance line. The aim of this study is to confirm the resistance gene of Dongnong 93-046 through a F8∶ 9populations from a cross between Dongnong 93-046 and Pin1246,to map the resistance gene,and to develop associated markers. The results showed resistance line number∶ susceptible line number is 1∶ 1 in the F8∶ 9generation,which showed that the resistance of Donong 93-046 was controlled by a pair of alleles. A total of 76 SSR molecular markers were screened between parents. A total of 13 SSR markers in linkage group F were used to construct a genetic map. The single marker analysis and composite interval analysis results show that a Dongnong 93046 resistance gene was located near the SSR marker Satt114. A total of 60 susceptible lines and 60 resistance lines were used to evaluate accuracy of the marker,the accuracy rate of this marker was80%. It offered a good foundation for molecular marker assisted selection.