五氯联苯PCB118对人肝癌细胞BEL-7402细胞黏附的影响

Effect of PCB118 on cell adhesion of human hepatocellular carcinoma cells BEL-7402

目的探讨五氯联苯PCB118对人肝癌细胞细胞-基质间和细胞-细胞间黏附的影响和机制。方法人肝癌细胞BEL-7402用PCB118 0.1,1.0和10.0 nmol·L^(-1)分别处理4和6 d后,采用细胞-基质间黏附实验检测细胞-基质间黏附,细胞聚集实验检测细胞间黏附,实时荧光定量PCR法和Western蛋白印迹法检测细胞黏附关键因子CD29、E-钙黏着蛋白和N-钙黏着蛋白的表达。结果与细胞对照组相比,人肝癌细胞BEL-7402在PCB118 0.1,1.0和10.0 nmol·L^(-1)处理6 d后,细胞-基质间黏附能力明显提高(P<0.05),细胞-细胞间黏附明显降低(P<0.05)。实时荧光定量PCR结果显示,PCB118明显提高黏附关键因子CD29和N-钙黏着蛋白m RNA水平(P<0.05),显著降低E-钙黏着蛋白m RNA水平(P<0.05)。Western蛋白印迹结果表明,PCB118处理后,CD29和N-钙黏着蛋白的蛋白表达显著升高(P<0.01),E-钙黏着蛋白的蛋白表达显著降低(P<0.01)。结论 PCB118影响肝癌细胞黏附并改变黏附因子CD29、E-钙黏着蛋白和N-钙黏着蛋白的表达,这可能与PCB118促进肝癌的发展有关。

OBJECTIVE To investigate the effects and mechanism of PCB118 on cell-matrix and cel-cel adhesion in human hepatocelular carcinoma cels. METHODS Human hepatocelular carcinoma cels BEL-7402 were treated with PCB118 0.1,1.0 and 10.0 nmol·L^- 1for 4 or 6 d,respectively. Then the cell- matrix adhesion assay and cell aggregation experiments were conducted to study the effect of PCB118 on cel-matrix adhesion and cel-cel adhesion in BEL-7402 cel s. Quantitative real-time PCR and Western blotting methods were employed to assess the expression of key cytokines CD29,N-cadherin and E-cadherin. RESULTS The results showed that the cell-matrix adhesion ability of human hepatocellular carcinoma cells BEL-7402 were significantly increased（P〈0.05）after treatment with PCB1180.1,1.0 and 10.0 nmol·L^- 1for 6 d,whereas the cell-cell adhesion ability was significantly reduced（P〈0.05）. Exposure to PCB118（0.1,1.0 and 10.0 nmol·L-1）for 6 d induced significant upregulation of the m RNA expression levels of CD29 and N-cadherin along with the downregulation of E-cadherin（P〈0.05）.Western blotting analysis revealed that PCB118 exposure significantly increased protein expressions of CD29 and N-cadherin but reduced E-cadherin protein level（P〈0.01）. CONCLUSION PCB118 exposure affects the expression of CD29,N-cadherin and E-cadherin, which may be involved in PCB118-induced alteration of cell adhesion of hepatocellular carcinoma cell line BEL-7402.