武汉地区缺血性脑卒中患者MTHFR基因多态性分布分析

Analysis of Polymorphism Distribution of MTHFR Gene in Ischemic Stroke Patients in Wuhan

目的:探讨武汉地区缺血性脑卒中患者亚甲基四氢叶酸还原酶(MTHFR)基因多态性的分布。方法:选取缺血性脑卒中患者167例为卒中组,健康体检者110例为对照组。通过基因芯片法检测缺血性脑卒中相关的MTHFR基因677位点,并按基因型别分为野生型(C677C)、杂合型(C677T)、纯合型(T677T)。结果:卒中组中野生型22.2%,杂合型43.1%,纯合型34.7%。与对照组相比,野生型显著减少,纯合突变型显著增多,有显著性差异(P<0.01)。不同性别脑卒中患者在MTHFR基因分型上差异无统计学意义(P>0.05)。结论:武汉地区缺血性脑卒中患者中分布有较多的纯合突变型MTHFR基因。

Objective：To analyze polymorphism distribution of methylenetetrahydrofolate reductase（MTHFR）gene in ischemic stroke patients in Wuhan. Methods： Total 167 cases of ischemic stroke patients were enrolled as stroke group, and 110 healthy cases were enrolled as control group. MTHFR genotypes were detected by the gene chip method, and for different genotypes of patients were divided into wild type（C677C）, heterozygous type（C677T） and homozygous type（T677T）. Results：In the stroke group, wild type accounted for 22.2%, heterozygous mutant type and homozygous mutant type accounted for 43.1%, 34.7%, respectively. Compared with the control group, the wild type was significantly reduced while the homozygous mutant was significantly increased（P〈0.05）. Different gender had no statistical significance in MTHFR genotype differences（P〈0.05）. Conclusion： There are more homozygous mutant MTHFR genes in ischemic stroke patients in Wuhan area.