早期针刺夹脊穴对MCAO大鼠脑纹状体?-内啡肽和强啡肽水平的影响

Effect of Early Acupuncture at Huatuo Jiajipoints on Striatal ?-endorphin and Dynorphin Levels in MCAO Rats

目的观察早期针刺夹脊穴对卒中后肢体痉挛大鼠脑纹状体?-内啡肽和强啡肽水平的影响。方法将70只SD大鼠随机分为A组(正常组)9只和B组(假手术组)10只,剩余大鼠制备卒中后肢体痉挛大鼠模型后随机分为C组(模型组)、D组(针刺夹脊穴组)和E组(巴氯芬组)。D组采用针刺夹脊穴治疗,E组采用巴氯芬片灌胃治疗。治疗7 d后,采用放射免疫方法检测各组动物脑纹状体?-内啡肽和强啡肽水平。结果与A组和B组比较,C组、D组和E组?-内啡肽水平均显著升高(P<0.01);与C组比较,D组和E组?-内啡肽水平均显著升高(P<0.01,P<0.05);D组?-内啡肽水平与E组比较,差异无统计学意义(P>0.05)。与A组和B组比较,C组、D组和E组内强啡肽水平均均显著升高(P<0.05,P<0.01);D组内强啡肽水平和E组比较,差异无统计学意义(P>0.05)。结论针刺夹脊穴能够增加卒中后肢体痉挛大鼠脑纹状体?-内啡肽水平而不能改变脑纹状体强啡肽水平,符合脑啡肽与痉挛关系,可改善动物的痉挛状态。

Objective To investigate the effect of early acupuncture at Huatuo jiaji points on striatal ？-endorphin and dynorphin levels in rats with post-stroke limb spasm. Methods Seventy SD rats were randomized into group A（normal） of 9 rats and group B（sham operation） of 10 rats. After a model of post-stroke limb spasm was made in the remaining rats, they were randomized into groups C（model）, D（acupuncture at Huatuo jiaji points） and E（baclofen）. Group D received acupuncture at Huatuo jiaji points and group E, an oral gavage of baclofen tablets. After seven days of treatment, striatal β-endorphin and dynorphin levels were neasured by radioimmunoassay. Results β-endorphin levels increased significantly in groups C, D and E compared with groups A and B（P〈0.01） and also in groups D and E compared with group C（P〈0.01,P〈0.05）. There was no statistically significant difference in β-endorphin levels between groups D AND E（P〈0.05）. Dynorphin levels increased significantly in groups C, D and E compared with groups A and B（P〈0.05,P〈0.01）. There was no statistically significant difference in dynorphin levels between groups D and E（P〈0.05）. Conclusions Acupuncture at Huatuo jiaji points can increase striatal β-endorphin levels but not change striatal dynorphin levels, which conforms to the relationship between enkephalin and spasm, and improve the animal＇s spasticity.