骆驼奶对2型糖尿病大鼠糖脂代谢及PPAR-γ、TNF-α mRNA的影响

Effects of camel milk on glycolipid metabolism and PPARγ、TNFα mRNA expression in rats of type 2 diabetes mellitus

目的研究骆驼奶(CM)对2型糖尿病大鼠体重、血糖、血脂、胰岛素,PPARγ和TNF-α基因表达的影响。方法采用高脂饲料加小剂量(30 mg/kg)链脲佐菌素(STZ)腹腔注射诱导2型糖尿病大鼠模型,将其分为4组,即正常对照(control)组、模型(model)组、骆驼奶低剂量(CM-L)组(3.5 mg/kg獉d)、骆驼奶高剂量(CM-H)组(10 mg/kg獉d),每周测定体重和血糖,第4周进行葡萄糖耐量实验,给药4周后断头处死,测定血脂(TC、TG、HDLC、LDL-C)、胰岛素水平,检测脂肪组织PPARγ和肝脏组织TNF-αmRNA表达量。结果与正常对照组相比,模型组大鼠体重明显下降(P<0.01),空腹血糖、糖耐量0、30、60、120 min血糖,以及血清TC、TG、LDL-C含量均显著增高(P<0.01),HDL-C含量下降,胰岛素水平升高。CM可缓解糖尿病大鼠体重下降,降低高血糖,CM-H组在给药第4周达到显著降糖效果,并显著降低糖耐量30 min血糖(P<0.05)。CM有降低糖尿病大鼠TC、TG、LDL-C含量,升高HDL-C含量,降低胰岛素趋势,其中CM-H剂量组可显著降低TG、LDL-C含量(P<0.05)。与正常对照组比较,模型组PPARγmRNA显著下调(P<0.05),TNF-αmRNA显著上调(P<0.01),CM使糖尿病大鼠的PPARγmRNA升高,其中,CM-H组差异显著(P<0.05),CM可降低其TNF-αmRNA表达量。结论 CM可缓解2型糖尿病大鼠体重下降,改善高血糖、糖耐量异常、血脂紊乱等症状,其机制可能与调节PPARγ、TNF-α表达有关。

Objective To investigate the effects of camel milk on body weight, blood glucose, lipid, insulin and FPAR-γ,TNF-α mRNA expressions in type 2 diabetes rats. Methods Type 2 diabetes was induced by high fat diet and small dose of STZ（i. g. 30 mg/kg） , rats were divided into four groups, normal control（control） group, diabetes model （model） group, camel-low dose（CM-L） group （3.5 mg/kg, d）, camel-high dose （CM-H） group （ 10 mg/kg· d）. Body weight and fasting blood glucose were measured every week. Glucose tolerance test（OGTT） was conducted at 4th week. After 4 weeks administration, animals were decapitated, plasma lipid （ TC, TG, HDL-C, LDL-C） , insulin were assayed.The expression of PPAR-7 and TNF-cx mRNA were measured using RT-PCR method. Results Compared with control group, the weight of model group lowered significantly（P 〈 0.01 ）, FBG and blood glucose at 0,30,60,120 min of OGTT, as well as the TC, TG, LDL-C concentrations increased（P 〈 0.01）, while HDL-C content reduced, and insulin level was higher than control rats. Compared with model group, body weight increased and blood glucose decreased in CM group, CM-H reduced the fasting glucose levels in 4th week and decreased the 30min glucose level significantly（ P 〈 0. 05）. CM lowered the TC, TG, LDL-C, insulin and increased HDL-C concentration, and it is significantly in CM-H（P 〈 0.05）. There is a marked decrease in PPAR~/ mRNA expression（P 〈 0.05） and a significant increase in TNF-α mRNA expression（P 〈 0.01 ）in diabetes rats. Compared with model rats, PPAR7 mRNA expression was ascended by CM administration, and it is notably in CM-H group（P 〈 0.05） , while the TNF-α mRNA expression of diabetes rats was lower, but there is no significant. Conclusions CM can improve the lowered body weight, hyperglycosemia, abnormal glucose tolerance and dyslipidemia in type 2 diabetes. The mechanism of these maybe associated with the regulation of PPAR7 and TNF-α mRNA.