盐度-光照强度-温度对小环藻Cyclotella sp. SHOU-B108生长及ARA和EPA含量的影响

Effects of salinity,light intensity and temperature on the growth,ARA and EPA contents in diatom Cyclotella sp. SHOU-B108

采用L_9(3~4)正交实验法,研究了盐度(3、6、9)、光照强度[20、40、60μmol/(m^2·s)]和温度(10、20、30℃)3因素对半咸水小环藻(Cyclotella sp.)SHOU-B108生长及细胞中ARA和EPA含量的影响。结果表明:培养10 d后小环藻在盐度6、光照强度40μmol/(m^2·s)和温度30℃组具有最大生物量(干重)。盐度、光照强度和温度对小环藻细胞ARA和EPA含量的影响均有显著的交互作用,但温度是影响藻细胞ARA和EPA含量的主要因素。低的盐度(3)和高的温度(30℃)有利于小环藻细胞积累ARA;而高的光照强度[60μmol/(m^2·s)]则有利于细胞积累EPA。本研究表明环境因子对小环藻细胞中不同的脂肪酸会产生不同的诱导效应,通过调控小环藻的培养条件,能够获得特定营养物质含量丰富的饵料微藻。

Effects of different levels of factors including salinity（ 3,6,9）,light intensity [20,40,60μmol/（ m^2· s） ] and temperature（ 10,20,30 ℃） on the growth,ARA and EPA contents in diatom Cyclotella sp. SHOU-B108 were studied by orthogonal experiments. The results showed that the highest biomass（ dry weight） of Cyclotella sp. was obtained in the treatment of salinity 6,light intensity 40 μmol/（ m^2· s） and temperature 30 ℃ on 10 th day. There were significant interactions among salinity,light intensity and temperature on the ARA and EPA contents in Cyclotella sp.. Temperature was the main factor influencing the ARA and EPA contents of the diatom. Lower salinity（ 3） combined with higher temperature（ 30 ℃） was beneficial to accumulating ARA but high light intensity（ 60 μmol/（ m2·s）） was beneficial for accumulating EPA in the cells of Cyclotella sp.. It is therefore suggested that different fatty acids in the cell display different reflections of the same environmental change,and Cyclotella sp. SHOU-B108 cells with specific high nutritional value could be harvested by optimizing the salinity-light intensity-culture temperature combinations.