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乳腺癌细胞中缺氧诱导因子-1α和miR-210的表达及其调控 被引量：1

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摘要 目的探讨乳腺癌MCF-7细胞中缺氧诱导因子(HIF)-1α与microRNA-210(miR-210)的表达情况及二者之间的调控关系。方法应用Western印迹和实时荧光定量聚合酶链反应(PCR)技术分别检测MCF-7细胞中HIF-1α和miR-210的表达情况。分别构建针对HIF-1α和miR-210的shRNA质粒表达载体,利用LipofectamineTM2000转染至MCF-7细胞中,观察HIF-1α和miR-210的表达情况。结果 MCF-7细胞中存在HIF-1α和miR-210表达,转染HIF-1α的shRNA后HIF-1α和miR-210的表达明显下降;转染miR-210的shRNA后HIF-1α表达没有变化,但miR-210的表达明显下降。结论乳腺癌MCF-7细胞中存在HIF-1α和miR-210表达,HIF-1α可正向调控miR-210的表达。
作者 蓝永洪 牛海艳 王晗 杨智 江朝娜 符碧薇 齐亚灵
机构地区 海南医学院
出处 《中国老年学杂志》 CAS CSCD 北大核心 2016年第11期2623-2625,共3页 Chinese Journal of Gerontology
基金 海南省自然科学基金(No.814301) 海南医学院科研培育基金(No.HY2013-12)
关键词 缺氧诱导因子-1Α microRNA-210 乳腺癌 调控关系
分类号 R730 [医药卫生—肿瘤]
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参考文献14

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中国老年学杂志
2016年 第11期

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