蒙药肋柱花在D-GlaN致急性肝损伤大鼠血清中移行成分的LC-MSDTrap分析

Analysis of Lomatogonium carinthiacum the frame column on D- GIa N to acute liver injury in rats serum transitional components by LC- MSDTrap

目的:通过分析肋柱花在D-氨基半乳糖(D-GIa N)致急性肝损伤大鼠血清中移行成分,探讨其肋柱花抗急性肝损伤药效物质基础。方法:以D-GIa N致急性肝损伤大鼠为研究对象、肋柱花水提物为示例药物,采用液相色谱/离子阱质谱(LC-MSDTrap)分析其肋柱花在D-GIa N致急性肝损伤大鼠血清中移行成分。结果:在D-GIa N致急性肝损伤大鼠血清中共检测到8个移行成分,其中1个移行成分为肋柱花原型成分、即獐牙菜苦苷,7个移行成分为代谢产物,通过二级质谱特征信息推测其化合物2、3、5、7、10的结构。结论:分析到的8个血中移行成分可能是肋柱花化学成分的体内作用形式,也最有可能成为肋柱花抗急性肝损伤的药效物质基础。

AIM To analyze Lomatogonium carinthiacum the frame column on D- GIaN to acute liver injury in rats serum transitional components,discuss the rib pillar against acute liver damage efficacy material base.METHODS： D- GIaN induced acute liver injury in rats as the research object and Lomatogonium carinthiacum water extract for example drugs,using LC- MSDTrap analysis of Lomatogonium carinthiacum in D- GIaN induced acute liver injury in rats serum transitional components. RESULTS： In the D- GIaN to acute liver injury in rats of serum was detected eight transitional elements,which is transitional component was prototype ingredients of Lomatogonium carinthiacum,namely Swertiamain,7 transitional components in metabolites,through secondary mass spectrometry speculates that the compound 2,3,5,7,10 of the structurecharacteristic information of the two stage mass spectrometry. CONCLUSION： The above 8 blood components may be the body function of the chemical composition of the carinthiacum Lomatogonium,also most likely to be the effective material basis for the anti acute liver injury of the carinthiacum Lomatogonium.