贵阳腐霉对致倦库蚊幼虫致病的组织学研究？

HISTOLOGICAL CHANGES OF CULEX PIPIENS QUINQUEFASCIATUS LARVAE INFECTED BY PYTHIUM GUIYANGENSE

为了观察灭蚊真菌贵阳腐霉感染致倦库蚊幼虫后的组织形态学变化，探讨贵阳腐霉对致倦库蚊幼虫的致死机理，采用石蜡切片、 H． E．染色和比较组织学方法，以未经贵阳腐霉作用的致倦库蚊幼虫为对照，连续观察用贵阳腐霉感染24～72 h致倦库蚊幼虫的组织学变化。结果发现被贵阳腐霉感染的致倦库蚊幼虫中肠上皮细胞出现细胞间隙增大，随着感染时间的延长，细胞间隙进一步增大并出现空泡化、质膜破损、细胞裂解一系列病理变化。结果表明贵阳腐霉可导致致倦库蚊幼虫中肠上皮细胞的破坏，推测这可能是导致其死亡原因之一。

In order to study the histological changes of Culex pipiens quinquefascitus larvae infected by this fungus and investigate the mechanism of Pythium guiyangense for killing Cx. quinquefascitus larvae. The larvae of Cx. quinquefasciatus at different infection periods were fixed. Their paraffin sections were made and stained with H. E. method, observed with microscope and photoed with NIS-Elements. The midgut of Cx. quinquefasciatus larvae were destructed remarkably, the gap of epithelial cells on midgut increases, cell vacuolation, the plasma membrane damage and cell lysis. It was considered as one of the lethal causes of Cx. quinquefasciatus larvae that P. guiyangense destroyed epithelial cells of midgut.