知母醇提物干预对2型糖尿病大鼠红细胞膜磷脂脂肪酸的影响

Anemarrhena Alcohol Extract Intervention on Type 2 Erythrocyte Membrane Phospholipid Fatty Acids in Diabetic Rats

目的:研究知母醇提物对2型糖尿病大鼠红细胞膜磷脂脂肪酸含量的影响。方法:采用气相色谱法测定衍生化的红细胞膜磷脂脂肪酸,用峰面积归一化法确定其相对含量,SPSS14.0软件进行独立样本t检验。结果:与空白组比较,知母醇提物高低剂量给药组与阳性对照药二甲双胍组C16∶0(软脂酸)、C18∶0(硬脂酸)、C18∶2(亚油酸)、C20∶4(花生四烯酸)的变化趋势一致,2型糖尿病模型组C18∶0、C18∶2、C20∶4的变化趋势与给药组相反,其中二甲双胍给药组、知母醇提物高剂量组C20∶4变化显著(P<0.05)。结论:知母醇提物可以通过影响2型糖尿病红细胞膜磷脂脂肪酸而发挥降糖作用。

Objective：The study on the content change of erythrocyte membrane phospholipid fatty acids of type 2diabetes under the the alcohol extract of Anemarrhenae intervention.Methods：Gas Chromatography derivatized erythrocyte membrane phospholipid fatty acids,and by peak area normalization method to determine the relative content,and independent sample t test with SPSS14.0.Results：Compared altogether alcohol extract low dose group and the positive control drug metformin group and the control group C16∶0,C18∶0,C18∶2,C20∶4in the same trend,type 2diabetes model group C18∶0,C18∶2,C20∶4trends and administration group 4of the contrary,which metformin administration group altogether alcohol extract high-dose group C20∶4changed significantly（P〈0.05）.Conclusion：Anemarrhena alcohol extract can type 2diabetes by affecting red blood cell membrane phospholipid fatty acids play a hypoglycemic effect.