螺旋藻激酶的体外抗氧化作用研究

Study on Anti-oxidative Effects of Spirulina Kinase in vitro

目的:研究螺旋藻激酶(SPK)的体外抗氧化作用。方法:采用邻二氮菲-Fe2+氧化法、1,1-二苯基-2-三硝基苯肼(DPPH)法和邻苯三酚自氧化法,分别测定不同浓度SPK对羟基(OH^-)自由基、DPPH自由基和对超氧阴离子(O_2^-)自由基的清除能力,并计算半数抑制浓度(IC_(50));采用普鲁士蓝反应法测定不同浓度SPK对Fe3+的还原能力(以吸光度值反映)。以上试验均以维生素C(VC)为阳性对照。结果:SPK对OH^-自由基、DPPH自由基及O_2^-自由基的清除能力均呈一定的浓度依赖性,SPK对OH^-自由基、DPPH自由基最大清除率分别86.82%、78.89%(IC_(50)分别为54.31、0.636 g/L),比VC(64.77%、73.49%)高;SPK对O_2^-自由基最大清除率为78.31%(IC_(50)为3.918 g/L),比VC(94.14%)低;在还原能力试验中,SPK使体系吸光度值增加,其最大吸光度值与VC相近,并呈明显浓度依赖性。结论:螺旋藻激酶具有明显的体外抗氧化活性。

OBJECTIVE：To study the anti-oxidative effects of spirulina kinase（SPK）in vitro.METHODS：The methods of phenanthroine-Fe^2＋oxidation method,DPPH and auto-oxidation of pyrogallol were used to measure the effects of different concentrations of SPK on scavenging hydroxyl（OH^-）free radical,DPPH free radical and superoxide anion（O2^-）free radical;IC50of SPK was calculated.Prussian blue reaction was used to determine total reducing ability（by absorbance）of different concentrations of SPK to Fe^3＋.Vitamin C（VC）was used as positive control in above trials.RESULTS：SPK could eliminate the OH^-free radical,DPPH free radical and O2^-free radical in concentration-dependant manner,and the maximum elimination rate of SPK to OH^-free radical and DPPH free radical were 86.82% and 78.98%（IC50were 54.31,0.636 g/L）,which were higher than VC（64.77%,73.49%）.The maximum elimination rate of SPK to O2^-free radical was 78.31%（IC50was 3.918 g/L）,which was lower than VC（94.14%）.In reducing ability test,SPK improved absorbance in reducing ability test system,and maximum absorbance was similar to VC in concentration-dependant manner.CONCLUSIONS：SPK has obvious anti-oxidant activities in vitro.