赤雹果总有机酸对寒凝血瘀模型大鼠凝血时间及血液流变学的影响

Effects of Total Organic Acid of Thladiantha dubia Fruit on Coagulation Time and Hemorheology of Rats with Cold Coagulation and Blood Stasis

目的:观察赤雹果总有机酸对寒凝血瘀模型大鼠凝血时间及血液流变学的影响。方法:将60只大鼠随机分为正常对照组(水)、模型对照组(水)、阿司匹林组(阳性药物,50 mg/kg)和赤雹果总有机酸低、中、高剂量组(50、100、200 mg/kg),每组10只。除正常对照组外,其余各组大鼠均采用4℃冰水浴冷刺激+ih肾上腺素复制寒凝血瘀模型,并同时ig相应药物,每天1次,连续14d。末次给药24 h后,腹主动脉取血,测定凝血时间、血细胞比容、血浆黏度、凝血酶原时间、凝血酶时间、活化部分凝血酶时间、血小板聚集率及全血低、中、高切黏度,并计算血沉方程K值。结果:与正常对照组比较,模型对照组凝血时间、凝血酶原时间、凝血酶时间、活化部分凝血酶时间缩短,血细胞比容、血浆黏度、血沉方程K值、血小板聚集率及全血低、中、高切黏度升高(P<0.05或P<0.01)。与模型对照组比较,阿司匹林组和赤雹果总有机酸中、高剂量组凝血时间、凝血酶时间及活化部分凝血酶时间延长,血小板聚集率降低;各给药组凝血酶原时间延长,血细胞比容、血浆黏度、血沉方程K值及全血低、中切黏度降低(P<0.05或P<0.01)。结论:赤雹果总有机酸对寒凝血瘀模型大鼠有明显的抗凝和改善血液流变学的作用。

OBJECTIVE：To observe the effects of total organic acid of Thladiantha dubia fruit（TOATF）on coagulation time and hemorheology of rats with cold coagulation and blood stasis.METHODS：60 rats were randomly divided in normal control group（water）,model control group（water）,aspirin group（positive drug,50 mg/kg）and TOATF low-dose,medium-dose and high-dose groups（50,100,200 mg/kg）with 10 rats in each group.Except for normal control group,cold coagulation and blood stasis model was induced by 4 ℃ water bath and subcutaneous injection of adrenaline hydrochloride,and then given correspondence medicine intragastrically,once a day,for consecutive 14 d.24 h after last administration,blood samples were collected from aorta abdominalis.The coagulating time（CT）,erythrocrit（HCT）,plasma viscosity（PV）,prothrombin time（PT）,thrombin time（TT）,activated partial thromboplastin time（APTT）,platelet aggregation rate（PAR）and whole blood low-shear,middle-shear and high-shear viscosity were measured and blood sedimentation equation K value was calculated.RESULTS：Compared with normal control group,CT,PT,TT and APTT of model control group were shortened,and HCT,PV,blood sedimentation equation K value,PAR and whole blood low-shear,middle-shear and high-shear viscosity increased（P〈0.05 or P〈0.01）.Compared with model control group,CT,PT and APTT prolonged in aspirin group and TOATF medium-dose and high-dose groups,and PAR decreased;PT of treatment groups prolonged,while HCT,PV,blood sedimentation equation K value and whole blood low-shear and middle-shear viscosity decreased（P〈0.05 or P〈0.01）.CONCLUSIONS：TOATF has obvious improvement effects on anticoagulation and hemorheology in rats with cold coagulation and blood stasis.