多指标评价不同干燥方法对北青龙衣质量的影响

Evaluation on the Effects of Different Drying Methods on the Quality of North Qinglongyi Based on Multiple Index

目的:研究不同干燥方法对北青龙衣质量的影响,从而优选北青龙衣的最优干燥方式。方法:对北青龙衣经5种干燥方法(阴干、晒干、烘干、微波干燥、冷冻干燥)及烘干方法下不同干燥温度(40、50、60℃)处理后,以干燥时间、复水率、含水量、醇浸出物含量、有效成分胡桃醌和胡桃酮的含量及其甲醇提取物对人胃腺癌BGC823细胞、人肺癌A549细胞的体外抗肿瘤活性为指标,对比分析不同干燥方法对北青龙衣质量的影响。结果:5种干燥方法中,微波干燥时间最短,阴干干燥时间最长;冷冻干燥样品含水量最大,微波干燥样品含水量最小;醇浸出物含量相差不大;胡桃醌含量以冷冻干燥样品最高、微波干燥样品最低;胡桃酮含量以40℃烘干样品最高、冷冻干燥样品最低;体外抗肿瘤试验表明按对2种细胞的生长抑制率将干燥方法排序为冷冻干燥>40℃烘干>阴干,且抑制率随着干燥温度的升高而降低。结论:不同干燥方法对北青龙衣质量有明显的影响。从成本、有效成分含量、抗肿瘤活性及实用性综合分析,认为北青龙衣宜采用在40℃烘干的方法进行干燥。

OBJECTIVE：To study the effects of different drying methods on the quality of North Qinglongyi,thus optimize the best drying way of North Qinglongyi.METHODS：North Qinglongyi was processed using five kinds of drying methods including drying in the shade,drying in the sun,oven drying,microwave drying,freeze drying and oven drying under different temperature（40,50,60 ℃）.The indicators were investigated,such as drying time,recovery rate of water,water content,alcohol extract,content of active ingredients walnut quinone and juglone,in vitro anti-tumor activity of methanol extract to human gastric adenocarcinoma BGC823 cells and human lung cancer A549 cells.The effects of different drying methods on the quality of North Qinglongyi were analyzed comparatively.RESULTS：Among 5 kinds of drying method,microwave drying time was the shortest,and the time of drying in the shade was the longest;water content of sample with freeze drying was the highest,and that with microwave drying was the lowest;the content of alcohol extract has little difference;the content of walnut quinone of sample with freeze drying was the highest,and that with microwave drying was the lowest;the content of juglone of sample dried at 40 ℃ was the highest,and that of freeze drying was the lowest;anti-tumor experiment in vitro showed that inhibition rate of sample with different drying methods on the growth of 2 kinds of cells was in descending order of freeze drying〉40 ℃ drying〉drying in the shade,and the inhibition rate decreased with the increase of drying temperature.CONCLUSIONS：Different drying methods have obvious influence on the quality of North Qinglongyi.40 ℃ drying method is the best for North Qinglongyi drying from the comprehensive analysis including cost,the contents of effective composition,anti-tumor activity and practice.