猪源多杀性巴氏杆菌的分离鉴定及荚膜血清分型

Isolation,identification and capsule serotyping of Pasteurella multocida originated from swine

为了解各血清型的多杀性巴氏杆菌(Pasteurella multocida,Pm)在四川、重庆、云南的流行情况,对2013—2014年间四川、重庆、云南等地8个规模化养殖场的125份病死猪肺炎样品进行细菌分离,并用PCR方法对分离的多杀性巴氏杆菌进行鉴定及荚膜血清分型。结果表明:从有肺炎症状病死猪的肺脏样品中共分离出11株多杀性巴氏杆菌,其中有7株为A血清型(63.6%),3株为D血清型(27.3%),1株为F血清型(9.1%),没有发现B和E血清型。表明在该区域内,猪肺疫主要是由A型Pm感染引起。另外,此次还分离出了1株较少见的F型多杀性巴氏杆菌。该结果可为上述地区巴氏杆菌病的防治提供科学材料。

To investigate the serotypes of Pasteurella multocida in Sichuan,Chongqing and Yunnan,a total of 125 tissue samples were collected from intensive piggery during 2013 to 2014. PCR was performed to identify the isolated Pasteurella multocida and separate their capsular serotyping. It was shown that 11 strains of Pasteurella multocida were isolated from the lung tissues of swine. PCR typing assay indicated that 7 strains were serotype A（ 63. 6%）,3strains were serotype D（ 27. 3%）,1 strain was serotype F（ 9. 1%）,the serotype B and serotype E were not found.It suggested that swine lung plague was mainly caused by serotype A Pasteurella multocida in these areas. In addition,a rare serotype F was isolated from the collected swine lung tissues. These results would provide scientific basis for the prevention and treatment of Pasteurella multocida in above areas.