摘要
目的:观察急性心肌梗死后大鼠心肌组织mi RNA-126表达水平的变化趋势以及疏血通注射液对急性心肌梗死大鼠心肌组织mi RNA-126表达水平的影响。方法:选取SD大鼠80只,结扎大鼠冠状动脉前降支,建立心肌梗死模型,存活60只,随机分为疏血通组及模型组,另随机选取30只大鼠作为对照组。建模成功后,疏血通组给予疏血通注射液治疗,模型组及假手术组予以等量生理盐水,采用实时定量PCR检测术后不同时间大鼠心肌组织mi RNA-126的表达水平变化。结果:术后第7天和第14天,疏血通组及模型组大鼠心肌组织mi RNA-126表达水平较假手术组明显降低(P<0.05),但2组间比较差异无统计学意义。术后第14天,疏血通组心肌组织mi RNA-126表达水平较前明显升高,差异有统计学意义(P<0.05)。结论:mi RNA-126在大鼠心肌梗死后7 d显著降低;随着时间的推移,mi RNA-126表达水平逐渐升高;疏血通能够上调mi RNA-126的表达水平。
Objective： To observe expression level change of miRNA-126 in the myocardial tissue of rats with myocardial infarction and to observe the influence of Shuxuetong injection. Methods： Myocardial infarction model was established in 80 SD rats, 60 survival rats were randomly divided into Shuxuetong group and model group, the other 30 rats were randomly selected as control group. After the success of the modeling, Shuxuetong group received Shuxuetong injection treatment, model group and control group received same amount of normal saline. Real-time quantitative PCR was used to detect miRNA-126 expression levels in rat myocardial tissue at direrent time after operation. Results： After 7 days and 14 days, miRNA-126 expression levels in rats myocardial tissue in Shuxuetong group and model group were significantly lower than that in the control group （P〈0. 05） , but there was no difference between the two groups. After 14 days, miRNA-126 expression levels in the Shuxuetong group were higher than that after 7 days （ P〈0. 05）. Conclusions： miRNA-126 in 7 days after myocardial infarction significantly reduced. With the passing of time, miRNA-126 expression levels increased gradually and Shuxuetong can increase the expression levels of miRNA-126 .
出处
《沈阳医学院学报》
2016年第3期146-148,共3页
Journal of Shenyang Medical College
基金
沈阳市科技局计划项目(No.F14-182-1-00/F14-158-9-26)
