叶绿素荧光动力学检测塑化剂对尖细栅藻的毒性效应

USE OF CHLOROPHYLL FLUORESCENCE TRANSITS TO ANALYSIS DEHP ON THE TOXICOLOGICAL EFFECTS OF SCENEDESMUS ACUMINATUS

以汉江水华藻类尖细栅藻Scenedesmus acuminatus为材料,研究了塑化剂(邻苯二甲酸二异辛酯,DEHP)对其光合系统的毒性效应。以不同浓度的DEHP处理S.acuminatus,分析其光合系统电子传递特征及相关参数,以及快速叶绿素荧光诱导动力曲线,发现低浓度的DEHP(10 mg/L)对S.acuminatus活性反应中心的电子传递链中的还原有促进作用,而高浓度(50—100 mg/L)则显著抑制;光合活性则与DEHP浓度呈效应-剂量关系,最大光化学效率降低;同时DEHP显著降低SOD活性,增加MDA含量,而且随DEHP浓度升高,处理时间的延长,效果越明显。研究结果表明,DEHP处理对S.acuminatus的光合系统传递链和抗氧化系统有一定的影响,说明快速荧光动力方法能够应用于DEHP毒性效应的快速检测。

To investigate the toxicological effects of DEHP on Scenedesmus acuminatus, a green algae isolated from Han River, chlorophyll fluorescence induction kinetics curve and the parameters and antioxidatant system were utilized. The results indicated that DEHP significantly decreased SOD activity but increased MDA content in a concentration and treatment time-dependent pattern, and that DEHP increased Chlorophyll contents at low concentration. Chlorophyll fluorescence induction kinetics curve and the parameters analysis showed that 10 mg/L DEHP promoted deoxidation of QA in the electron transport chain, and 100 mg/L DEHP reduced the deoxidation of QA. DEHP decreased Fv/Fm with the increased concentration and the duration time. The results revealed that DEHP has an obvious damage on the photosynthetic system and antioxidant system of S. acuminatus, and fast chlorophyll fluorescence induction kinetics （OJIP-test） can be effectively used to detect detrimental damage.