维生素D对急性肺水肿小鼠的保护作用及机制研究

Protective effect of vitamin D on acute lung edema mice and its mechanism

目的探讨维生素D与急性肺水肿小鼠之间的关系,以明确其在急性肺水肿中的作用及机制。方法用脂多糖腹腔注射方法制作急性肺水肿小鼠模型,给予雄性BALB/c小鼠活性维生素D类似物帕立骨化醇腹腔注射2周,设立空白及模型对照组,分析各组小鼠生存时间,观察小鼠肺组织湿质量/干质量比,肺系数,肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6水平,髓过氧化物酶(MPO)的活性,并做肺组织病理切片。结果维生素D处理组肺水肿小鼠相对于对照组生存时间延长(P<0.05);病理损伤改善,湿质量/干质量比,肺系数有所降低;TNF-α、IL-6水平,MPO的活性均降低(P<0.01或P<0.05)。结论维生素D能够缓解急性肺水肿小鼠的病理改变,其机制可能与减少炎症因子释放有关。临床上应用维生素D治疗可能会减轻患者的肺水肿。

Objective To explore the relationship between vitamin D and acute pulmonary edema in mice,and to investigate its effects in the process of acute pulmonary edema.Methods Acute lung edema mouse model was established by i.p.injection LPS.BALB/c male mice were treated with active vitamin D analogue paricalcitol（every other day i.p.injection）for 2weeks.To analyze the survival time,wet-dry ratio,lung index,the level of TNF-α,IL-6,the activity of MPO and histological examination in lung tissues.Results Vitamin D treated lung edema mice showed higher survival time than the vehicle-treated controls（P0.05）.The pathological injury was reduced by vitamin D.The wet-dry ratio and lung index was lower in vitamin D-treated mice compared with control group.The levels of TNF-α,IL-6and the activity of MPO were significantly down-regulated by vitamin D（P0.01 or P0.05）.Conclusion These observations suggest that vitamin D could improve the pathological changes in acute pulmonary edema mice,which may be related to the reduced release of inflammatory factors.The clinical application of vitamin D therapy may ameliorate pulmonary edema of patients..