摘要
目的 研究支气管肺泡灌洗液 (BALF)中端粒酶检测对肺癌的诊断价值。方法 收集 63例原发性肺癌和 3 1例非肺癌肺疾病患者BALF ,采用TRAP银染法检测其端粒酶。同步行经纤维支气管镜刷片病理学、灌洗液脱落细胞学检查。结果 肺癌组端粒酶阳性检出率为 76.2 % (4 8/ 63 ) ,高于对照组的 6.5 %(2 / 3 1) (χ2 =40 .6,P <0 .0 1) ,且高于刷检的阳性率 (5 8.7% ,3 7/ 63 ) (χ2 =3 .6,P >0 .0 5 ) ,也高于BALF细胞学的阳性率 (14 .3 % ,9/ 63 ) (χ2 =46.3 ,P <0 .0 1)。中心型肺癌组端粒酶阳性率为 71.4% (3 5 / 49) ,高于BALF细胞学的阳性率 (12 .2 % ,6/ 49) (χ2 =3 5 .3 ,P <0 .0 1) ,但与刷检 61.2 % (3 0 / 49)的阳性率间无明显差异 (χ2 =1.1,P >0 .0 5 )。周围型肺癌组BALF中端粒酶阳性率为 92 .9% (13 / 14 ) ,高于刷检的阳性率 (5 0 .0 % ,7/ 14 ) ,也高于BALF细胞学的阳性率 (2 1.4% ,3 / 14 )。结论 检测肺部疾病患者BALF中的端粒酶对确诊肺癌有一定的价值。
Objective To investigate the diagnostic significance of detection of telomerase activity of bronchoalveolar lavage liquid (BALF) in primary lung cancer. Methods BALF of 63 patients with lung cancer and 31 patients with non cancerous pulmonary disease was collected, and telomerase activity was detected with silver stained TRAP. Brush cytology and BALF cytology were carried out simultaneously. Results The positive rate of telomerase was 76.2% (48/63) in lung cancer group, which was higher than 6.5% (2/31) in control group (χ 2=40.6, P<0.01). The positive rate was also higher than that of brush cytology (58.7%, 37/63) (χ 2 =3.6, P>0.05), and higher than that of BALF cytology (14.3%, 9/63) (χ 2=46.3, P<0.01). In central type lung cancer group, the positive rate of telomerase was 71.4% (35/49), and higher than that of BALF cytology (12.2%, 6/49) (χ 2= 35.3 , P<0.01), but had no significant difference compared to that of brush cytology (61.2%, 30/49) (χ 2=1.1, P>0.05). In peripheral type lung cancer group, the positive rate of telomerase in BALF was 92.9% (13/14), and higher than that of brush cytology (50.0%, 7/14) and that of BALF cytology (21.4%, 3/14). Conclusion The telomerase activity could be detected in the BALF of the primary lung cancer. Hence, detection of telomerase in the BALF of patients may be helpful to diagnose lung cancer.
出处
《中国肺癌杂志》
CAS
2002年第4期275-277,共3页
Chinese Journal of Lung Cancer
