UPLC法快速测定栀子复方制剂中栀子苷和西红花苷-1含量

Simultaneous determination of crocin-1 and geniposide in Zhizi preparations by UPLC

目的建立快速测定栀子复方制剂复方牛黄消炎胶囊、清胃黄连丸和龙泽熊胆胶囊中栀子苷和西红花苷-1的方法。方法采用UPLC法。色谱柱为BEH C18柱(50mm×2.1mm,1.7μm);流动相:甲醇-水以不同梯度洗脱;流速:0.3mL·min^(-1);检测波长:0~7min为238nm,7~20min为440nm;柱温:30℃;进样量为2μL。结果 3种复方制剂中栀子苷在0.010~1.000mg·mL^(-1)范围内呈良好的线性关系(r=0.999 9),平均回收率分别为98.2%,99.9%和100.3%,RSD分别为1.3%,1.4%和1.0%;西红花苷-1在0.1~10.0μg·mL^(-1)范围内呈良好的线性关系(r=0.999 9)。平均回收率分别为99.6%,97.4%和98.3%,RSD分别为1.3%,1.4%和1.5%。结论栀子苷和西红花苷-1在20min内获得良好分离,该方法简便、准确、快速,可用于栀子复方制剂中栀子苷和西红花苷-1的测定。

Objective To establish a fast method for simultaneous determination of geniposide and crocin-1in three kinds of traditional Chinese medicines by UPLC.Methods UPLC was adopted in this study.The analytical column was BEH C18（50 mm×2.1mm,1.7μm）and gradient elution with methanol-H_2O as mobile phase.The flow rate was 0.3mL·min^-1.The detection wavelength was set at 238nm（0-7min）for geniposide and 440nm（7-20min）for crocin-1.The column temperature was 30 ℃and the injection volume was 2μL.Results The two components had a good linear relationship between 0.010-1.000mg·mL^-1and 0.1-10.0μg·mL^-1.The average recoveries（n=6）of geniposide and crocin-1in three preparations were 98.2%,99.9%and100.3%（RSD 1.3%,1.4% and 1.0%）and 99.6%,97.4% and 98.3%（RSD 1.3%,1.4% and 1.5%）,respectively.Conclusion The separation of geniposide and crocin-1only needs 20 min with accuracy and good reliability.The method is simple,specific and suitable for the simultaneous determination of the content of geniposide and crocin-1in the three preparations.