微波消解-ICP-OES法测定水獭肝中的铁、锌、铜和镁含量

Content determination of four kinds of heavy metal elements in Lutralutra linnaeus by microwave digestion and ICP-OES

目的建立水獭肝中Fe、Zn、Cu和Mg 4种无机元素含量的分析方法。方法采用微波消解电感耦合等离子体原子发射光谱法（ICP-OES）法测定水獭肝中4种重金属元素,用国家生物成分分析标准物质黄芪验证方法的准确度。结果该方法线性良好,相关系数均≥0.999 9,相对标准偏差（n=6）≤2.0%,回收率在97%~103%之间,标准物质黄芪的测定值与标准值基本吻合。结论该方法快速、准确、灵敏度高,可以用于水獭肝中的铁、锌、铜、镁的测定。

Objective To develop a method for the determintation of four inorganic elements（Fe,Zn,Cu and Mg）in Lutralutra linnaeus.Methods Samples were digested by microwave digestion system and determined by inductively coupled plasma atomic emission spectrometry（ICP-OES）.The method was evaluated by the analysis of certified reference materials for the chemical composition of biological samples（astragalus）.Results The method had good linear correlation（r≥0.999 9）.The average recoveries were in the range of 97%-103% with RSD less than 2.0%.The results obtained showed close agreement with the national standard substances of astragalus.Conclusion The method is fast,accurate and reliable,which can be used to determine the content of Fe,Zn,Cu and Mg in Lutralutra linnaeus.