摘要
目的了解2011~2014年我院临床分离菌的分布和耐药特性,为临床合理使用抗菌药物提供参考。方法采用纸片扩散法（K-B法）对我院分离的菌株进行药敏试验并对肠杆菌科细菌进行ESBL检测,按照CLSI 2012年标准判定药敏结果,用WHONET 5.6软件进行数据分析。结果 2011~2014年我院共分离细菌7 178株,其中革兰阴性杆菌5 831株,革兰阳性球菌1 347株。大肠埃希菌和金黄色葡萄球菌分别占首位。大肠埃希菌ESBLs的检出率在55%~66%,肺炎克雷伯菌ESBLs的检出率逐年上升,鲍曼不动杆菌对大部分抗菌药物的耐药率超过60%,铜绿假单胞菌的耐药上升相对平稳,葡萄球菌属MRSA的检出率高于地区平均水平,未检出耐万古霉素菌株。结论我院住院患者分离的细菌耐药性呈逐年上升趋势,应根据药敏检测结果合理使用抗菌药物。
Objective To investigate the distribution and antibiotic resistance profile of clinical isolated bacteria in our hospital during2011-2014.Method Kirby-Bauer method was used to conduct susceptibility testing and ESBLs detection.The results were analyzed by WHONET 5.6software according to CLSI 2010.Result A total of 7 178 becteria were isolated from 2011 to 2014in our hospital in which 5 831 were Gram-negative bacilli and 1 347 were Gram-positive cocci.E.coli and Staphylococcus aureus were the most commom pathogens.ESBLs detection rate of E.coli was 55%-66%.ESBLs detection rate of Klebsiella sppincreased year by year.The resistance rate of Acinetobacter sppto most antimicrobials was more than 60%.Resistance of pseudomonas aeruginosa raised relatively stable,Staphylococcus MRSA detection rate was higher than the regional average.Vancomycin-resistant strains were not detected.Conclusion Antimicrobial resistance in clinical bacterial isolates showed a serious situation in our hospital.Antimicrobial agents should be used rationally based on the results of susceptibility testing.
出处
《西北药学杂志》
CAS
2016年第3期319-323,共5页
Northwest Pharmaceutical Journal
关键词
细菌耐药性
革兰阴性杆菌
革兰阳性球菌
bacteria resistance
Gram-negative bacilli
Gram-positive cocci
