杜香中黄酮类化学成分的分离与鉴定

Chemical Constituents of Flavonoids in Ledum Palustre L.

目的:研究杜香(Ledum palustre L.)嫩枝中的黄酮类化学成分。方法:通过硅胶、ODS等柱色谱和制备型HPLC对其进行分离,采用1H和13C-NMR谱,结合HRESI-MS质谱数据鉴定化合物结构。结果:分离得到5个黄酮类化合物,分别鉴定为licuraside(1)、芦丁(2)、槲皮素-3-O-β-D-木糖基-(1→2)-β-D-半乳糖苷(3)、金丝桃苷(4)、异鼠李素-3-O-(6-香豆酸酯)-β-D-葡萄糖苷(5)。结论:化合物3和5为首次从杜香属植物中分离得到。

Objective： To study the chemical constituents of flavonoids in Ledum palustre L. Methods： Flavonoids were isolated and purified from ethyl acetate extracts of Ledum palustre L. by chromatography on silica gel and ODS column chromatography and HPLC. Their structures were identified on the basis of the spectroscopy of HRESI- MS,1H and13C- NMR. Result： Five flavonoids were isolated and identified as licuraside（ 1）,Rutin（ 2）,quercetin- 3- O- β- D- xylopyranosyl（ 1 →2）- β- D- galactopyranoside（ 3）,hyperoside（ 4） and isorhamnetin- 3- O-（ 6- coumaroyl）- β- D- glucopyranoside（ 5）. Conclusion： Compounds 3and 5 are obtained from the plants in Ledum L. for the first time.