摘要
目的:建立骨松停胶囊的质量标准。方法:采用薄层色谱法(TLC)进行本方中的骨碎补、杜仲、淫羊藿、女贞子、狗脊的定性鉴别;采用高效液相色谱法(HPLC)进行骨碎补中主要有效成分柚皮苷的含量测定。结果:在TLC色谱中,样品斑点清晰,与其他成分分离度好;HPLC色谱中,柚皮苷峰可与其他成分峰有效分离,在0.286~2.288μg范围内与色谱峰面积呈现良好的线性关系,相关系数0.9998,平均回收率99.2%。结论:所建立的方法可以准确地进行定性、定量检测,可用于该制剂的质量控制。
Objective: To establish the quality standard of Gusongting capsules. Methods: Drynariae Rhizoma,Eucommiae Cortex,Epimedii Folium,Ligustri Lucidi Fructus,Cibotii Rhizoma were identified by TLC; the contents of Naringin were determined by HPLC. Results: The TLC sample spots were clear and separate; the HPLC method showed that the peak of Naringin could be effective for the separation of other component peaks,with chromatographic peak area in the range of 0. 286 ~ 2. 288 μg,which presented a good linear relationship,with a correlative coefficiency of 0. 9998. The average recovery rate was 99. 2%. Conclusion: The method is accurate and can be used for the quality control.
出处
《中医药信息》
2016年第3期37-40,共4页
Information on Traditional Chinese Medicine
基金
黑龙江省应用技术研究与开发计划项目(No.GC13C108)
关键词
骨松停胶囊
鉴别
薄层色谱法
含量测定
高效液相色谱法
Gusongting capsules
Identification
Thin layer chromatography
Content determination
HPLC