UCP2基因rs660339多态性与壮族儿童青少年单纯性肥胖的相关性分析

Association of UCP2 gene rs660339 single nucleotide polymorphism with simple obesity in Chinese Zhuang children adolescents

目的探讨UCP2基因位点rs660339单核苷酸多态性与广西柳州的壮族儿童青少年单纯性肥胖的相关性。方法随机选取285名柳州市儿童青少年（年龄3~18岁）,其中单纯性肥胖组151例,健康对照组134例,分别检测身高和体重,测定空腹血糖（fasting blood-glucose,FBG）、血清总胆固醇（total cholesterol,TC）、甘油三酯（triacylglyceride,TG）、高密度脂蛋白胆固醇（high density lipoproteincholesterol,HDL-C）和低密度脂蛋白胆固醇（low density lipoprotein-cholesterol,LDL-C）两组标本UCP2基因rs660339位点利用SNa Pshot技术检测,统计基因型及等位基因分布频率,并分析两组等位基因构成比。结果肥胖组中除年龄、身高外,与对照组的差异均有统计学意义（P〈0.05）。健康对照组和肥胖组UCP2基因rs660339 C/C型、C/T型及T/T型基因型频率分别为40.30%、49.25%、10.45%和37.09%、50.33%、12.58%,两组C、T等位基因频率分别为64.93%、35.07%和62.25%、37.75%,T等位基因的OR值为1.122,95.9%的可信区间为0.797~1.580。基因型与等位基因频率差异均无统计学意义（P〉0.05）。rs660339位点TT与CC/CT基因型与各代谢指标（血糖、总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白）无显著相关性。结论广西壮族儿童青少年单纯性肥胖组和健康对照组UCP2基因单核苷酸多态性位点rs660339位点基因型的构成比组间无显著性差异,各基因型携带者间代谢指标也无显著性差异。

Objective To investigate the relationship of UCP2 gene rs660339 polymorphisms on obesity in Chinese Zhuang children and adolescents. Methods 285 cases were enrolled in the study,including 151 obese children and adolescents and 134 normal weights as the control. Height and weight of all the participants were collected. Fasting blood-glucose（FBG）,total cholesterol（TC）,triacylglyceride（TG）,high density lipoprotein-cholesterol（HDL-C）and low density lipoprotein-cholesterol（LDL-C）were detected.The genotype of rs660339 were typed by SNa Pshot technique. Statistical analysis of genotype frequencies between obese participants and healthy controls was performed using the SPSS program. Results The metabolic parameters（FBG,TC,TG,HDL-C and VDL-C）of the obese group were significantly different when compared to that of the control group. The C/C,C/T,T/T genotype frequencies of UCP2 rs660339 in simple obese group and normal control group were 40.30%,49.25%,10.45% and 37.09%,50.33%,12.58%,respectively. The C,T allelic frequencies in simple obese group and normal control group were 64.93%,35.07% and 62.25%,37.75%,respectively. Odds ratio（OR）is 1.122（CI：0.797-1.580）. The genotype and allelic frequencies of rs660339 in simple obese children were not significantly different from those in controls（P0.05）,and there was no significant association between rs660339 and obestity- related traits including FBG,TC,TG,HDL-C and VDL-C. Conclusion rs660339 polymorphism in UCP2 gene is not associated with simple obese in Chinese Zhuang children in Liuzhou,Guangxi province,and there was no significant association among the genotypes and metabolic parameters.