CD27＋B细胞在乙型肝炎病毒相关性疾病患者外周血中的变化及其临床意义

The changes of CD27＋ B cells in peripheral blood of the patients with HBV related disease and its clinical significance

目的观察乙型肝炎病毒（HBV）相关性疾病不同严重程度患者外周血CD27＋B细胞百分率的变化及其临床意义。方法选取慢性乙型病毒性肝炎20例、乙肝相关性肝硬化23例、肝癌21例，同期健康体检者25例设为健康对照组，分别收集健康对照组及HBV相关性疾病患者外周血，以流式细胞术检测细胞免疫水平变化：CD27＋B细胞百分率的值；免疫透射比浊法检测体液免疫：IgM、IgD表达的变化。结果（1）与健康对照组比较，三组HBV相关性疾病患者CD27＋B细胞百分率明显下降[（5．16±0．36）％比（4．52±0．22）％、（2．24±0．15）％、（0．58±0．02）％，F=4．32，P〈0．05]，并且随着疾病程度的加重其下降的趋势逐渐明显；（2）三组HBV相关性疾病患者IgM、IgD明显上升，并且随着疾病程度的加重其升高的幅度愈加明显（F=3．29、5．23，P=0．02、0．03）。结论CD27＋B细胞与HBV相关性疾病具有密切关系，重建机体免疫防御系统对评估HBV相关性疾病预后及指导临床有重要意义。

Objective To observe the changes of peripheral blood CD27＋ B cells percentage in patients with HBV - related disease of different severity and the clinical significance. Methods 20 cases of chronic viral hepatitis B ,23 cases of HBV - related liver cirrhosis ,21 cases of liver cancer were selected ,25 cases of healthy controls in the corresponding period who had the physical examination were set as the healthy control group. The peripheral blood in the healthy controls and the patients with HBV - related disease was collected. The cellular immune level changes and CD27＋B cells percentage was detected by flow cytometry, the humoral immunity was detected by immune transmission turbidty method：IgM, IgD. Results （1）Compared with the healthy control group, CD27 B cells percentage significantly decreased in the three groups of patients with HBV - related disease[ （5.16 ±0.36） % vs. （4.52 ±0.22） %, （2.24 ± 0.15 ） %, （0.58 ± 0.02 ） %, F = 4.32, P 〈 0.05 ], and the downward trend gradually obvious as the disease degree exacerbated, nnnnn（2）IgM, IgD in the three groups of HBV related disease patients rised obviously, and the increase range became more obvious as the disease degree exacerbation （ F = 3.29,5.23, P = 0.02,0.03 ）. Conclusion CD27＋ B cells has a close relationship with HBV -related disease, and rebuilding the body＇s immune defense system is great importance for evaluating prognosis and the clinical guidance in HBV -related disease.