类风湿关节炎患者病损关节滑膜组织高迁移率族蛋白1表达和凋亡水平与疾病活动性的关系

Relation of HMGB1 expression and cell apoptosis of synovial tissue with disease activity parameters in rheumatoid arthritis patients

目的探讨类风湿关节炎(RA)关节滑膜细胞凋亡水平及高迁移率族蛋白1(HMGB1)表达变化与RA临床疾病活动性指标之间的相关关系。方法采用TUNEL细胞凋亡实验、免疫组织化学染色法分别检测12例RA患者和12例骨关节炎(OA)患者的滑膜组织HMGB1表达水平和细胞凋亡水平,同时采用免疫速率散射比浊法、酶联免疫吸附试验(ELISA)以及红外线障碍法分别检测血清C反应蛋白(CRP)、类风湿因子(RF)、抗环瓜氨酸抗体(抗CCP)和红细胞沉降率(ESR)水平。结果 RA组和OA组之间年龄和性别构成差异均无统计学意义(P>0.05)。RA组表达CRP、RF、抗CCP和ESR水平均明显高于OA组(P<0.01)。RA组病损关节滑膜组织HMGB1水平明显高于OA组(P<0.01),而细胞凋亡水平则明显低于OA组(P<0.01)。RA患者关节滑膜组织凋亡水平评分与CRP、ESR、抗CCP、RF水平均呈强负相关(r值分别为-0.628、-0.815、-0.783和-0.757,P<0.05),而HMGB1表达水平评分与CRP、ESR、抗CCP、RF水平均呈强正相关(r值分别为0.638、0.739、0.698和0.648,P<0.05)。结论 RA患者关节滑膜细胞高表达HMGB1,可能通过减少细胞凋亡影响RA疾病的活动性。

Objective To investigate the relation of high mobility group box-1 protein（HMGB1）expression and cell apoptosis of synovial tissue with disease activity parameters in rheumatoid arthritis（RA）patients. MethodsTUNEL apoptosis assay and immunochemistry analysis were used to determine HMGB1 expression and cell apoptosis of synovial tissue in RA patients（12 cases）and osteoarthritis（OA） patients（12 cases）. Immune rate scatter nephelometry,enzyme-linked immunosorbent assay（ELISA）and infrared barrier method were performed to determine serum C-reactive protein（CRP）,rheumatoid factor（RF）,anti-cyclic citrullinated peptide（CCP）antibody and erythrocyte sedimentation rate（ESR）of the 2 groups. Results The differences for age and sex between RA and OA groups were not statistically signifi cant（P〉0.05）. CRP,RF,anti-CCP antibody and ESR were signifi cantly higher in RA group than those in OA group（P〈0.01）. HMGB1 expression in synovial tissue was signifi cantly higher in RA group than those in OA group（P〈0.01）,and the cell apoptosis of synovial tissue was signifi cantly lower in RA group than that in OA group（P〈0.01）. The score of cell apoptosis in RA group was negatively correlated with CRP,ESR,antiCCP antibody and RF（r=-0.628,-0.815,-0.783 and-0.757,P〈0.05）,and HMGB1 expression score was positively correlated with CRP,ESR,anti-CCP antibody and RF（r=0.638,0.739,0.698 and 0.648,P〈0.05）. ConclusionsHMGB1 expression in RA group might affect the disease activity of RA by regulating cell apoptosis.