两种小鼠心脏骤停模型方法的建立和比较

Comparisonof the Effect of the Two Methods to Induce Cardiac Arrest in Mouse

目的:比较经食道心室起搏及经静脉注射氯化钾建立小鼠心脏骤停模型的疗效,为深入开展心肺复苏研究提供模型基础。方法:20只小鼠随机分为两组(n=10):经食道快速起搏心室致心脏骤停组(起搏组)和经静脉注射氯化钾组(氯化钾组),起搏组为经食道快速起搏心室诱发室颤建立心脏骤停模型,刺激4 min时开始行常规心肺复苏。氯化钾组则通过静脉注射氯化钾建立小鼠心脏骤停模型,5 min时开始行常规心肺复苏。自主循环恢复的小鼠连续监测心电和血压60 min,观察血压、心率学及生存时间。结果:两组小鼠全部诱发心脏骤停成功,两组小鼠自主循环恢复率相当(9/10,8/10,P>0.05)。氯化钾组小鼠的生存时间长于起搏组,但差异无统计学意义。结论:采用经食道起搏与经静脉注射氯化钾均可建立小鼠心脏骤停模型。但氯化钾组小鼠心脏骤停时间长于起搏组,而生存时间也长于起搏组,推测氯化钾具有保护作用而不适合进一步干预实验研究。经食道快速起搏致心脏骤停模型更适合后续实验研究。

Objectlve：To compare the transesophageal cardiac pacing and intravenous injection of potassium chlorideto inducing cardiac arrest （CA） model,to provide the experimental basis for the further researches of cardiopulmonary resuscitation （CPR）. Methods： 20 mice were randomly divided into transesophageal cardiac pacing group （pacing group, n=10）and intravenous injection of potassium chloride group（potassium chloride group,n=10）. Transesophageal cardiac pacing was performed to elicit ventricular fibrillation in 10 mice. Four minutes after onset of cardiac arrest, conventional CPR was initiated. Cardiac arrest was induced by intravenous injection of potassium chloride and five minutes after onset of cardiac arrest conventional CPR was initiated.Continuous monitoring of electrocardiogramand blood pressure in mice after restoration of spontaneous circulation （ROSC）in one hour,and survival time were observed in two groups. Results：Cardiac arrest was induced successfully in both groups. The rates of ROSC were similar between two groups （9/10,8/10, P〉0.05 ）.The survival time was longer in potassium chloride group than that of pacing group, however, there was no significant difference. Conclusions： Either cardiac pacing or potassium chloride could induce CA successfully. The time of CA was longer in potassium chloride group than that of pacing group, the survival time also was longer. Potassium chloride might improve the effect of CPR. Transesophageal cardiac pacing is more appropriate to induce CA for the further study of CPR.