公共场所从业人员和综合医院肺炎患者嗜肺军团菌尿抗原和血清抗体检测

Detection of antigen in urine and antibody in serum of Legionella pneumophila in employees from public buildings and pneumonia patients from general hospitals

目的调查公共场所从业人员和综合医院肺炎患者嗜肺军团菌尿抗原和血清抗体的阳性情况。方法于2012—2014年选择华东两城市集中空调系统嗜肺军团菌培养法阳性的7家公共场所从业人员（n=280）和华北、华东和华南共4城市的7家综合医院肺炎患者（尿样：n=262;血样：n=269）为研究对象,采集人群的尿和血清样本,以酶联免疫吸附法检测尿中嗜肺军团菌抗原和血清嗜肺军团菌抗体。结果从业人员嗜肺军团菌尿抗原和血清抗体阳性率为10.4%和36.1%,肺炎患者嗜肺军团菌尿抗原和血清抗体阳性率为11.8%和18.2%。从业人员嗜肺军团菌尿抗原和血清抗体阳性检出率差异有统计学意义（χ^2=7.133,P=0.000）,肺炎患者嗜肺军团菌尿抗原和血清抗体阳性检出率差异无统计学意义（χ^2=0.291,P=0.794）。结论本次调查的公共场所从业人员和综合医院肺炎患者均存在嗜肺军团菌感染。

Objective To investigate the positive rate of antigen in urine and antibody in serum of Legionella pneumophila in employees from public buildings and pneumonia patients from general hospitals. Methods The urine and serum samples were collected from employees in seven public buildings and pneumonia patients in seven general hospitals in four cities from 2012 to 2014. Legionella pneumophila antigen in urine and antibody in serum were detected by ELISA. Results The positive rate of Legionella pneumophila antigen in urine and Legionella pneumophila antibody in serum of employees were 10.4% and 36.1% respectively. The positive rate of Legionella pneumophila antigen in urine and Legionella pneumophila antibody in serum of pneumonia patients were 11.8% and 18.2% respectively. There was significant difference in the positive rate of Legionella pneumophila antigen in urine and antibody in serum of employees（ χ~2=7.133,P=0.000）. There was no significant difference in the positive rate of Legionella pneumophila antigen in urine and antibody in serum of pneumonia patients（ χ~2=0.291,P=0.794）.Conclusion There are Legionella pneumophila infection in employees of public buildings and pneumonia patients of general hospitals in this survey.