敲低肌切蛋白可抑制胃肠道间质瘤细胞的增殖能力

Knockdown of scinderin inhibits proliferation in gastrointestinal stromal tumor cells

目的探讨肌切蛋白（scinderin,SCIN）对胃肠道间质瘤（gastrointestinal stromal tumor,GIST）细胞增殖能力的影响。方法用shRNA慢病毒干扰技术将SCIN在GIST细胞中稳定敲低,并通过Western blot和qRT-PCR检测敲低效率。通过CCK-8实验检测伊马替尼IC50和SCIN对GIST细胞增殖能力的影响,通过平板克隆形成实验检测SCIN对GIST细胞克隆形成能力的影响,通过裸鼠皮下成瘤实验检测SCIN对移植瘤生长的影响。实验按照对GIST细胞进行的不同处理分组：WT组（野生型组,未进行转染）,Mock组（阴性对照组,转染阴性对照序列）,sh SCIN 1组（转染SCIN干扰序列1）,sh SCIN 2组（转染SCIN干扰序列2）。结果 sh SCIN 1组和sh SCIN 2组GIST细胞在增殖能力上明显低于Mock组,增殖能力在第2~7天均有明显差异（n=4,P〈0.05）。移植瘤实验中sh SCIN 1组较Mock组的移植瘤质量显著降低：sh SCIN 1组vs Mock组=（0.175 0±0.045 6）g vs（0.334 1±0.016 5）g（n=4,P〈0.01）。平板克隆形成实验显示sh SCIN 1组克隆形成数显著少于Mock组,分别为59.67±5.68和123.33±2.52（n=3,P〈0.01）。在使用0.57μmol/L的伊马替尼处理WT组细胞1、3、5 d后,SCIN的表达随处理时间而降低。结论敲低SCIN可以抑制GIST细胞在体外和体内环境中的增殖能力,伊马替尼可以抑制GIST细胞中SCIN的表达,SCIN可能成为GIST的治疗靶点。

Objective To determine the role of scinderin（ SCIN） in regulating proliferation of gastrointestinal stromal tumor（ GIST） cells. Methods SCIN-knockdown GIST cells were established with SCIN-specific shRNAs（ sh SCINs）. The knockdown efficiency was determined by Western blotting and qRTPCR. CCK-8 assay was used to evaluate imatinib IC50 and the effect of sh SCIN on proliferation. Plate colony formation assay was used to test the effect of sh SCINs on colony formation of GIST cells. Xenograft assay in nude mice was employed to test the effect of sh SCINs on tumor growth. According to different treatment,GIST cells were divided into 4 groups： wild type group（ WT group,no transfection）,mock group（ transfected with scrambled siRNA）,sh SCIN1 group（ transfected with sh SCIN1） and sh SCIN 2 group（ transfected with sh SCIN2）. Results The cell proliferation of sh SCIN1 group and sh SCIN2 group was significantly decreased compared with the mock group. The proliferation difference was significant from the second day to the seventh day（ n = 4,P 0. 05）. Xenografts derived from sh SCIN1 cells had significantly lower tumor weight than those derived from mock cells（ 0. 1750 ± 0. 0456 vs 0. 3341 ± 0. 0165 g,n = 4,P 0. 01）. The colony numbers derived from sh SCIN1 cells and mock cells were 59. 67 ± 5. 68 and 123. 33 ± 2. 52,respectively（ n = 3,P 0. 01）. After treatment with 0. 57 μmol / L imatinib in WT cells for 1,3 and 5 d,the expression of SCIN was found to be decreased in a time-dependent manner. Conclusion Knockdown of SCIN inhibits the proliferation of GIST cells both in vitro and in vivo. Imatinib inhibits the expression of SCIN. SCIN may be a promising therapeutic target for GIST.